Nowak-Sliwinska Patrycja, Karocki Andrzej, Elas Martyna, Pawlak Anna, Stochel Grazyna, Urbanska Krystyna
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
Biochem Biophys Res Commun. 2006 Oct 20;349(2):549-55. doi: 10.1016/j.bbrc.2006.08.060. Epub 2006 Aug 22.
The efficiency of photodynamic effect (PDE) for Photofrin II (PfII), Verteporfin, and Merocyanine 540 (MC540) was compared against neoplastic cells. Triplet state lifetimes and singlet molecular oxygen quantum yields were correlated with biological effect. PfII triplet lifetime was two times longer than that of Verteporfin, however, its singlet molecular oxygen quantum yield was two times lower in comparison with Verteporfin. High singlet molecular oxygen quantum yield of Verteporfin resulted in high biological efficacy. To achieve 50% mortality of cells four times lower light dose and five times lower concentration of Verteporfin were applied in comparison with PfII. The same level of cell damage was reached using 10 times higher light dose and two times higher concentration of MC540 in comparison with PfII. Our results confirm that singlet molecular oxygen based mechanism, prevalent for Verteporfin and PfII, was highly effective against melanoma cells. Verteporfin can be used at small doses with high cellular damage efficiency.
将二血卟啉醚（PfII）、维替泊芬和部花青540（MC540）的光动力效应（PDE）效率与肿瘤细胞进行了比较。三重态寿命和单线态分子氧量子产率与生物学效应相关。PfII的三重态寿命比维替泊芬长两倍，然而，与维替泊芬相比，其单线态分子氧量子产率低两倍。维替泊芬的高单线态分子氧量子产率导致了高生物学功效。与PfII相比，达到50%细胞死亡率时，维替泊芬的光照剂量低四倍，浓度低五倍。与PfII相比，使用高十倍的光照剂量和高二倍的MC540浓度可达到相同程度的细胞损伤。我们的结果证实了以单线态分子氧为基础的机制，这在维替泊芬和PfII中普遍存在，对黑色素瘤细胞非常有效。维替泊芬可以小剂量使用，细胞损伤效率高。
