Chaichana Kaisorn, Zamora-Berridi Grettel, Camara-Quintana Joaquin, Quiñones-Hinojosa Alfredo
Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
Stem Cells. 2006 Dec;24(12):2851-7. doi: 10.1634/stemcells.2006-0399. Epub 2006 Aug 31.
The "no new neuron" dogma that the brain is quiescent throughout adult life has been challenged by the discovery of cells with stem cell-like qualities of self-renewal and multipotency in the subventricular zone of the lateral ventricles and the dentate gyrus of the hippocampus in adults. This self-renewing capacity also makes these neural stem cells a possible source of brain tumors, which was supported by the discovery of self-sustaining brain tumor stem-like cells in cancers such as glioblastoma multiforme. Neurosphere assays are the standard for studying these stem-like cells in both normal and cancer tissues. Despite the importance of these assays, there is no standardized protocol to allow for a comparison of results because several studies use different growth factors and hormones at different concentrations. The primary objective of this study is to review the literature for both nontumor and tumor studies to assess their respective neurosphere assay components. We found significant variation in assay components, namely hormones and growth factors, as well as their respective concentrations. This illustrates the need for a standardized protocol to allow proper comparison among studies and a better assessment of the effects of different factors.
“无新神经元”这一观点，即大脑在整个成年期都处于静止状态，已受到挑战。人们发现，在成年人侧脑室的室下区和海马体的齿状回中，存在具有自我更新和多能性等干细胞样特性的细胞。这种自我更新能力也使这些神经干细胞成为脑肿瘤的一个可能来源，多形性胶质母细胞瘤等癌症中自我维持的脑肿瘤干细胞样细胞的发现支持了这一点。神经球分析是研究正常组织和癌组织中这些干细胞样细胞的标准方法。尽管这些分析很重要，但由于几项研究使用不同浓度的不同生长因子和激素，所以没有标准化方案来进行结果比较。本研究的主要目的是回顾非肿瘤和肿瘤研究的文献，以评估它们各自的神经球分析成分。我们发现分析成分，即激素和生长因子，以及它们各自的浓度存在显著差异。这表明需要一个标准化方案，以便在研究之间进行适当比较，并更好地评估不同因素的影响。
