Mori H, Ishida-Yamamoto A, Senba E, Ueda Y, Tohyama M
Department of Anesthesiology, Osaka Dental University, Japan.
J Chem Neuroanat. 1990 May-Jun;3(3):155-63.
Differential peptidergic innervation of the rat buccal mucosa and incisor tooth pulp was demonstrated by experimental immunohistochemistry. In the buccal mucosa, calcitonin gene-related peptide (CGRP)-like immunoreactive (IR) nerve fibres were observed in both the epithelium and submucosal layer. Most of these fibres were also immunoreactive to substance P (SP). On the other hand, SP-IR fibres were less numerous than CGRP-IR fibres in the incisor tooth pulp. Fast blue (FB) was applied to the buccal mucosa and the incisor pulp chamber. Small (less than 20 microns) FB-labelled CGRP-IR neurons were more numerous in the buccal mucosa group, while medium to large neurons (20-50 microns) predominated in the incisor pulp. Following neonatal capsaicin treatment, CGRP- and SP-containing fibres in the buccal mucosa almost totally disappeared, but many CGRP-IR fibres remained in the incisor tooth pulp. The findings suggest that these two oral tissues are innervated by different subgroups of CGRP-IR neurons in the trigeminal ganglia.
通过实验性免疫组织化学方法证实了大鼠颊黏膜和切牙髓的肽能神经支配存在差异。在颊黏膜中，上皮层和黏膜下层均观察到降钙素基因相关肽（CGRP）样免疫反应性（IR）神经纤维。这些纤维大多也对P物质（SP）呈免疫反应性。另一方面，在切牙髓中，SP-IR纤维比CGRP-IR纤维数量少。将快蓝（FB）应用于颊黏膜和切牙髓腔。在颊黏膜组中，小（小于20微米）的FB标记CGRP-IR神经元数量较多，而在切牙髓中，中到大神经元（20 - 50微米）占主导。新生大鼠经辣椒素处理后，颊黏膜中含CGRP和SP的纤维几乎完全消失，但切牙髓中仍有许多CGRP-IR纤维。这些发现表明，这两种口腔组织由三叉神经节中不同亚组的CGRP-IR神经元支配。
