Kerezoudis N P, Olgart L, Fried K
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Histochemistry. 1993 Oct;100(4):319-22. doi: 10.1007/BF00270053.
In this study we examined the presence and localization of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity in the dental pulp, periodontal tissues and alveolar bone of the rat. The presence of NADPH-d activity was also examined in cat pulp. The rat histochemical analysis revealed the presence of prominent NADPH-d activity both in cells of the sub-odontoblastic cell layer and in the odontoblasts, in the root as well as in the coronal pulp regions. In the pulpal horns, odontoblasts often had long processes with a high level of labelling indicating NADPH-d activity extending through the predentin and dentin. Moreover, endothelial cells of pulpal blood vessels were positive for NADPH-d in both species. However, no clearcut examples were found of pulpal nerve fibres positive for NADPH-d in the rat or cat and denervation performed in rats did not alter the enzyme staining patterns. In the periodontal tissue, NADPH-d activity was localized to cells on the alveolar bone surface of the periodontal ligament and, in addition, alveolar bone marrow crypts were filled with intensely labelled cells. In the gingival papillae, NADPH-d activity was observed in the basal cell layer of the epithelium. Endothelial cells of periodontal and gingival blood vessels showing positive staining for NADPH-d were occasionally noted.
在本研究中，我们检测了大鼠牙髓、牙周组织和牙槽骨中烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）活性的存在及定位情况。同时也检测了猫牙髓中NADPH-d活性的存在情况。大鼠组织化学分析显示，在成牙本质细胞下层细胞和成牙本质细胞中，以及在牙根和冠部牙髓区域均存在显著的NADPH-d活性。在牙髓角处，成牙本质细胞常常具有长突起，且标记水平较高，表明NADPH-d活性穿过前期牙本质和牙本质延伸。此外，两种动物的牙髓血管内皮细胞NADPH-d均呈阳性。然而，在大鼠或猫中未发现明确的牙髓神经纤维NADPH-d呈阳性的例子，并且对大鼠进行去神经支配并未改变酶染色模式。在牙周组织中，NADPH-d活性定位于牙周膜牙槽骨表面的细胞，此外，牙槽骨骨髓隐窝中充满了强阳性标记细胞。在牙龈乳头中，在上皮基底层观察到NADPH-d活性。偶尔会注意到牙周和牙龈血管的内皮细胞NADPH-d染色呈阳性。
