Bahraoui E, Benjouad A, Sabatier J M, Allain J P, Laurian Y, Montagnier L, Gluckman J C
Laboratoire de Biochimie CNRS UA 1179, Faculté de Médecine, Marseille, France.
Blood. 1990 Jul 1;76(1):257-64.
The relevance, previously suggested by some authors, of anti-nef antibody detection as an early marker of human immunodeficiency virus (HIV) infection was examined with a sensitive liquid phase radioimmunoassay by investigating: (1) the kinetics of appearance of anti-nef-antibodies in a set of 77 longitudinal sera collected from 12 HIV-infected donors at the time of seroconversion; and (2) nef serology in a population of 32 HIV seropositive and three seronegative hemophiliacs and their seronegative or seropositive sexual partners. The results obtained showed that anti-nef antibodies could not be detected in the sera tested independently of the appearance of antibodies specific to HIV structural proteins. Thus, the detection of anti-nef antibodies appears to be of little diagnostic value.
一些作者之前曾提出抗Nef抗体检测作为人类免疫缺陷病毒（HIV）感染早期标志物的相关性，本研究采用灵敏的液相放射免疫测定法对此进行了检验，具体研究内容如下：（1）在一组从12名HIV感染供体血清转化时收集的77份纵向血清中，检测抗Nef抗体出现的动力学情况；（2）检测32名HIV血清阳性和3名血清阴性血友病患者及其血清阴性或血清阳性性伴侣群体中的Nef血清学情况。所得结果表明，在所检测的血清中，无论HIV结构蛋白特异性抗体是否出现，均未检测到抗Nef抗体。因此，抗Nef抗体检测似乎几乎没有诊断价值。
