Cheingsong-Popov R, Panagiotidi C, Ali M, Bowcock S, Watkins P, Aronstam A, Wassef M, Weber J
Department of Infectious Diseases, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
AIDS Res Hum Retroviruses. 1990 Sep;6(9):1099-105. doi: 10.1089/aid.1990.6.1099.
A sensitive and specific enzyme-linked immunoassay for antibodies to the human immunodeficiency virus type 1 (HIV-1) nef gene product, p27, has been developed using recombinant Escherichia coli-derived protein from the LAV-1-Bru sequence. Of 92 HIV-1 infected hemophiliacs, 72 (78%) produced anti-nef antibodies in this assay; the early appearance of anti-nef prior to full seroconversion was a rare event in this population, occurring in only one subject (approximately 1%). Anti-nef antibodies were not detected in any of 500 sera from 98 repeatedly HIV seronegative subjects who had been exposed to sexually transmitted modes of HIV infection (45 subjects) or through blood products (53 subjects). There was no significant association of titer or anti-nef antibody with protection from disease in HIV infection (p = 0.1). Although the nef protein is relatively immunogenic in natural infection, this study cannot confirm the previously reported high prevalence of anti-nef antibodies prior to seroconversion, nor the finding of anti-nef antibodies in HIV seronegative but exposed subjects.
利用来自LAV-1-Bru序列的重组大肠杆菌衍生蛋白，开发了一种用于检测针对人类免疫缺陷病毒1型（HIV-1）nef基因产物p27抗体的灵敏且特异的酶联免疫测定法。在92名感染HIV-1的血友病患者中，72名（78%）在此测定法中产生了抗nef抗体；在该群体中，完全血清转化前抗nef抗体的早期出现是罕见事件，仅在一名受试者中出现（约1%）。在98名反复HIV血清学阴性的受试者的500份血清中未检测到抗nef抗体，这些受试者通过性传播方式（45名受试者）或血液制品（53名受试者）接触过HIV感染。在HIV感染中，抗体滴度或抗nef抗体与疾病保护之间无显著关联（p = 0.1）。尽管nef蛋白在自然感染中具有相对免疫原性，但本研究无法证实先前报道的血清转化前抗nef抗体的高流行率，也无法证实HIV血清学阴性但已接触过HIV的受试者中存在抗nef抗体这一发现。
