血清无培养基培养中下丘脑细胞从色氨酸合成血清素。

Serotonin synthesis from tryptophan by hypothalamic cells in serum-free medium culture.

作者信息

Becquet D, Francois-Bellan A M, Boudouresque F, Faudon M, Hery F, Guillaume V, Hery M

机构信息

Faculté de Médecine Nord, Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297, Marseille, France.

出版信息

Brain Res Dev Brain Res. 1990 Jun 1;54(1):142-6. doi: 10.1016/0165-3806(90)90075-a.

DOI:10.1016/0165-3806(90)90075-a

PMID:1694745

Abstract

The hypothalamus of both adult and fetal rats contains a population of cells which can exhibit some features of serotoninergic (5-HT) neurons under certain circumstances. However, their neuronal serotoninergic nature is still controversial. In fact the presence of tryptophan hydroxylase activity has not yet been clearly established. This study attempted to verify whether [3H]5-HT can be synthesized from [3H]tryptophan ([3H]TRP) in hypothalamic cell cultures from 16-day-old fetuses. Data showed that [3H]5-HT was synthesized from [3H]TRP and the amounts of [3H]5-HT increased linearly as a function of time for 60 min. Pargyline markedly increased the quantities of [3H]5-HT and decreased those of [3H]5-hydroxyindole acetic acid. [3H]5-HT synthesis was inhibited by p-chlorophenylalanine, while alpha-methyl-p-tyrosine had no effect. The present biochemical study shows the presence of an intrinsic 5-HT neuronal system in the hypothalamus of the fetal rat.

摘要

成年大鼠和胎鼠的下丘脑均含有一群细胞，在某些情况下，这些细胞可表现出血清素能（5-羟色胺，5-HT）神经元的一些特征。然而，它们的神经元血清素能性质仍存在争议。事实上，色氨酸羟化酶活性的存在尚未得到明确证实。本研究试图验证在来自16日龄胎儿的下丘脑细胞培养物中，[3H]5-羟色胺能否由[3H]色氨酸（[3H]TRP）合成。数据表明，[3H]5-羟色胺由[3H]色氨酸合成，且[3H]5-羟色胺的量在60分钟内随时间呈线性增加。优降宁显著增加了[3H]5-羟色胺的量，并减少了[3H]5-羟吲哚乙酸的量。[3H]5-羟色胺的合成受到对氯苯丙氨酸的抑制，而α-甲基对酪氨酸则无作用。目前的生化研究表明，胎鼠下丘脑存在一个内在的5-羟色胺能神经元系统。