Kwok Y N, McIntosh C H
Department of Physiology, University of British Columbia, Vancouver, Canada.
Eur J Pharmacol. 1990 May 16;180(2-3):201-7. doi: 10.1016/0014-2999(90)90303-n.
The release of gastric substance P-like immunoreactivity (SP-LI) has been studied in the vascularly perfused rat stomach. In the presence of 20 microM bacitracin and captopril, basal release of SP-LI was sustained throughout the experiments. Gastric SP-LI release was stimulated in a concentration-dependent manner by increasing the concentration of KCl in the perfusion medium. This stimulated release was reduced by the omission of Ca2+, indicating that a Ca2(+)-dependent mechanism was involved. Naloxone did not alter basal SP-LI secretion. [Met5]Enkephalin also had no significant effect on K(+)-stimulated secretion suggesting that enkephalinergic mechanisms are not involved. Gastric SP-LI release was also increased by capsaicin perfusion but this was not sustained. In conclusion, the present results provide the first evidence for the release of SP-LI into the rat stomach vasculature.
已在血管灌流的大鼠胃中研究了胃P物质样免疫反应性（SP-LI）的释放。在存在20微摩尔杆菌肽和卡托普利的情况下，整个实验过程中SP-LI的基础释放持续存在。通过增加灌流介质中氯化钾的浓度，胃SP-LI释放以浓度依赖性方式受到刺激。这种刺激释放因去除Ca2+而减少，表明涉及一种Ca2+依赖性机制。纳洛酮不改变SP-LI的基础分泌。[Met5]脑啡肽对K+刺激的分泌也无显著影响，提示不涉及脑啡肽能机制。辣椒素灌流也增加胃SP-LI释放，但这种增加不持久。总之，本研究结果首次为SP-LI释放到大鼠胃血管系统中提供了证据。
