Gordeeff M, Clergeau-Guerithault S
Faculté de Chirurgie Dentaire, Nantes.
J Biol Buccale. 1990 Mar;18(1):59-67.
The histopathologic diagnosis of odontogenic cysts is based mainly on the morphological nature of the epithelial lining of the cyst. A standard immunocytochemical method based on anticytokeratin monoclonal antibodies was used for the diagnosis of dentigerous and primordial cysts: 12 odontogenic cysts were diagnosed on clinical, radiological and pathological criteria in 9 dentigerous cysts and 3 primordial cysts. The anticytokeratin antibodies used in this study were KL1 (Immunotech, France) and AE1, AE2, AE3 and AE8 (ICN-Miles, France). The anticytokeratin antibodies used stained only the epithelial cells confirming their accuracy. The KL1 antibodies stained homogeneously the various epithelial cells. This positive reaction was not modified by the various fixation methods used. Some reactions observed with AE antibodies seemed to be modified by Bouin's fixative. The staining homogeneity of the primordial cysts and the staining heterogeneity of the dentigerous cysts seemed to be related to morphological aspects of their respective epithelia. The epithelial reactions in these 2 types of cysts towards inflammation were different.
牙源性囊肿的组织病理学诊断主要基于囊肿上皮衬里的形态学特征。一种基于抗细胞角蛋白单克隆抗体的标准免疫细胞化学方法被用于诊断含牙囊肿和始基囊肿：根据临床、放射学和病理学标准诊断出12例牙源性囊肿，其中9例为含牙囊肿，3例为始基囊肿。本研究中使用的抗细胞角蛋白抗体为KL1（法国Immunotech公司）以及AE1、AE2、AE3和AE8（法国ICN-Miles公司）。所使用的抗细胞角蛋白抗体仅对上皮细胞染色，证实了其准确性。KL1抗体对各种上皮细胞进行均匀染色。这种阳性反应不会因所使用的各种固定方法而改变。使用AE抗体观察到的一些反应似乎会被Bouin固定液改变。始基囊肿的染色均匀性和含牙囊肿的染色异质性似乎与它们各自上皮的形态学方面有关。这两种类型囊肿的上皮对炎症的反应不同。
