Volpes R, van den Oord J J, Desmet V J
Department of Pathology, University Hospital St. Rafaël, Catholic University of Leuven, Belgium.
J Hepatol. 1990 May;10(3):297-304. doi: 10.1016/0168-8278(90)90136-f.
The distribution of the thrombospondin-receptor was studied in 26 cases of inflammatory hepatitis B virus (HBV) related liver disease and twelve non-inflammatory controls using monoclonal antibody (mcab) OKM5. OKM5 reacted in all cases with sinusoidal lining cells, portal vessel endothelium, and scattered mononuclear inflammatory cells. In 16 of the 26 cases with inflammation, one or more clusters of OKM5 positive (OKM5+) hepatocytes were found in areas of periportal or intralobular inflammation, whereas the remaining ten cases did not show hepatocellular OKM5 reactivity. In all but one case, OKM5+ liver cells coexpressed HLA-DR-antigens, and in twelve cases cytotoxic/suppressor T-cells were enriched in the OKM5+/HLA-DR+ liver cell clusters, suggesting induction of OKM5 positivity by lymphokines released by nearby T-cells. In view of its role in cytoadherence, it is suggested that OKM5 expression by hepatocytes serves in the entrapment and retention of inflammatory cells thereby facilitating their activation. Furthermore, OKM5+/HLA-DR+ hepatocytes might trigger an autologous mixed lymphocyte reaction (AMLR), resulting in down-modulation of ongoing hepatic inflammatory responses.
使用单克隆抗体（mcab）OKM5研究了26例炎症性乙型肝炎病毒（HBV）相关肝病患者和12例非炎症对照中血小板反应蛋白受体的分布。OKM5在所有病例中均与窦状隙衬里细胞、门静脉血管内皮细胞和散在的单核炎症细胞发生反应。在26例炎症患者中的16例中，在门周或小叶内炎症区域发现一个或多个OKM5阳性（OKM5+）肝细胞簇，而其余10例未显示肝细胞OKM5反应性。除1例病例外，所有OKM5+肝细胞均共表达HLA-DR抗原，并且在12例病例中，细胞毒性/抑制性T细胞在OKM5+/HLA-DR+肝细胞簇中富集，提示附近T细胞释放的淋巴因子诱导了OKM5阳性。鉴于其在细胞粘附中的作用，提示肝细胞表达OKM5有助于捕获和保留炎症细胞，从而促进其活化。此外，OKM5+/HLA-DR+肝细胞可能引发自体混合淋巴细胞反应（AMLR），导致正在进行的肝脏炎症反应下调。
