García Monzón C, García Buey L, García Sánchez A, de Castro Marinas M, Muñoz Gómez R, Pajares J M, González Mateos F, Moreno Otero R
Unidad de Hepatología (Servicio de Digestivo), Hospital de la Princesa, Universidad Autónoma, Madrid.
Rev Esp Enferm Dig. 1993 Nov;84(5):301-9.
To analyze the expression of different HLA class I antigens and cell adhesion molecules on frozen liver sections from patients with chronic active hepatitis, types B and C.
Liver biopsy samples were divided into two parts, one for routine histological examination and another, after snap-freezing and storing at -80 degrees C, for immunohistochemical analysis. To carry out immunoperoxidase and immunofluorescence stainings, a panel of monoclonal antibodies specific for HLA class I light (beta 2-microglobulin) and heavy chain determinants, and for adhesion molecules such as intercellular adhesion molecule-1 and lymphocyte function associate antigen-3 was used.
Immunohistochemistry was performed in frozen liver biopsy sections from 25 patients with viral chronic active hepatitis, 10 type B and 15 type C. As controls, normal liver samples and liver specimens from patients with cholestasis or steatosis were also studied.
HLA class I light and heavy chain determinants were expressed on hepatocytes, biliary duct epithelium, sinusoidal lining cells and lymphocytes from patients and controls; however, the beta 2-microglobulin conformational epitope was undetectable on hepatocytes from normal livers or with cholestasis or steatosis, but clearly positive on hepatocytes from patients with hepatitis. In addition, in liver sections from controls no adhesion molecules positivity was detected on hepatocytes; by contrast, hepatocytes from patients with hepatitis showed markedly enhanced membranous reactivity for both adhesion molecules in areas of piecemeal and lobular necrosis.
Virus B and C infections could induce an increased hepatocellular expression of HLA-class I determinants, including the conformational epitope adequate for antigen presentation, and cell-cell adhesion molecules. These findings underline the role of cell mediated immune reactions in the pathogenesis of hepatic injury in viral chronic hepatitis.
分析慢性乙型和丙型活动性肝炎患者冷冻肝切片中不同HLA I类抗原和细胞黏附分子的表达情况。
肝活检样本分为两部分，一部分用于常规组织学检查，另一部分在速冻并储存于-80℃后用于免疫组化分析。为进行免疫过氧化物酶和免疫荧光染色，使用了一组针对HLA I类轻链（β2-微球蛋白）和重链决定簇以及细胞间黏附分子-1和淋巴细胞功能相关抗原-3等黏附分子的单克隆抗体。
对25例病毒性慢性活动性肝炎患者（10例乙型和15例丙型）的冷冻肝活检切片进行免疫组化。作为对照，还研究了正常肝样本以及胆汁淤积或脂肪变性患者的肝标本。
HLA I类轻链和重链决定簇在患者和对照的肝细胞、胆管上皮细胞、窦状隙衬里细胞和淋巴细胞上表达；然而，正常肝脏或胆汁淤积或脂肪变性患者的肝细胞上未检测到β2-微球蛋白构象表位，但肝炎患者的肝细胞上明显呈阳性。此外，对照的肝切片中未检测到肝细胞上有黏附分子阳性；相比之下，肝炎患者的肝细胞在碎片状坏死和小叶坏死区域对两种黏附分子均表现出明显增强的膜反应性。
乙型和丙型病毒感染可诱导肝细胞HLA I类决定簇表达增加，包括适合抗原呈递的构象表位以及细胞间黏附分子。这些发现强调了细胞介导的免疫反应在病毒性慢性肝炎肝损伤发病机制中的作用。
