Niu Jia-feng, Zhang Cai, Zhang Jian-hua, Xu Xiao-qun, Zhang Wei-dong, Wang Jun-fu, Gao Chun-yi
Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory for Tumor Immunity and Genetic Engineering of Shandong Province, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Sep;22(5):557-9.
To investigate the effect of IFN-alpha on expression of MHC class I chain-related protein A (MICA) in the cervical carcinoma cell lines.
The cervical carcinoma cell lines (HeLa and Caski) were treated with IFN-alpha. The expression of MICA was measured by RT-PCR and by immunohistochemical staining. The cytotoxicity of human NK cells to the IFN-alpha treated cervical carcinoma cells was detected by MTT method.
The mRNA and protein expression of MICA was up-regulated by IFN-alpha in HeLa and Caski cells in a time and dose-dependent manner. Compared to Caski cells which weakly expressed MICA, higher cytolytic activity of NK cells was found against HeLa cells, which expressed relatively higher level of MICA. After being treated with IFN-alpha for 3 d, the susceptibility of the two cervical carcinoma cells to NK cytolysis was increased significantly.
IFN-alpha can up-regulate the MICA expression in the cervical carcinoma cell lines and thereby enhance the susceptibility to cytolysis of NK cells.
研究α干扰素对宫颈癌细胞系中MHC I类链相关蛋白A（MICA）表达的影响。
用α干扰素处理宫颈癌细胞系（HeLa和Caski）。通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学染色检测MICA的表达。采用MTT法检测人自然杀伤（NK）细胞对经α干扰素处理的宫颈癌细胞的细胞毒性。
α干扰素在HeLa和Caski细胞中以时间和剂量依赖性方式上调MICA的mRNA和蛋白表达。与弱表达MICA的Caski细胞相比，NK细胞对表达相对较高水平MICA的HeLa细胞具有更高的细胞溶解活性。经α干扰素处理3天后，两种宫颈癌细胞对NK细胞溶解的敏感性显著增加。
α干扰素可上调宫颈癌细胞系中MICA的表达，从而增强对NK细胞溶解的敏感性。
