Liu Ji-fang, Liu Feng, Qi Yan-chao, Yang Bo, Lu Min-ying, Pan Dong-xiao, Shen Hong-zhuo
Laboratory Center of the Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou 510095, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Apr;26(4):354-5, 358.
To explore IFN-gamma-mediated reversion of CIK killing sensitivity to immunoedited lung cancer A549 cells.
RT-PCR and MTT methods were used to detect the effect on MICA mRNA expression induced by IFN-gamma in the edited A549 cells and the change of CIK killing sensitivity to A549 cells, respectively.
Low expression of cell surface MICA and low killing sensitivity of CIKs were observed in edited A549 cells. IFN-gamma could significantly increase MICA mRNA expression in the edited A549 cells and improve CIK cell cytotoxicity.
IFN-gamma could reverse CIK killing sensitivity to the edited A549 cells by enhancing the MICA mRNA expression.
探讨γ干扰素介导的CIK对免疫编辑肺癌A549细胞杀伤敏感性的逆转作用。
分别采用逆转录聚合酶链反应（RT-PCR）和四甲基偶氮唑蓝（MTT）法检测γ干扰素对编辑后的A549细胞中MICA信使核糖核酸（mRNA）表达的影响以及CIK对A549细胞杀伤敏感性的变化。
在编辑后的A549细胞中观察到细胞表面MICA低表达以及CIK杀伤敏感性低。γ干扰素可显著增加编辑后的A549细胞中MICA mRNA表达并提高CIK细胞的细胞毒性。
γ干扰素可通过增强MICA mRNA表达来逆转CIK对编辑后的A549细胞的杀伤敏感性。
