槲皮素抑制人胃癌细胞生长并诱导其凋亡

[Quercetin inhibits growth and induces apoptosis of human gastric carcinoma cells].

作者信息

Wang Hai-yan, Guo Liang-miao, Chen Yong, Zhao Xue-hua, Cheng Cai-lian, Wu Mian-yun, He Li-ya

机构信息

Institute of Cellular and Molecular Biology, College of Medical Science, Wuhan University of Science and Technology, Wuhan 430080, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Sep;22(5):585-7.

PMID:16948901

Abstract

AIM

To study the effect of quercetin on the growth and apoptosis of human gastric carcinoma cell line MGC-803.

METHODS

The measurement of inhibitory rate and apoptotic index(AI) of quercetin were done by MTT assay and TUNEL assay. The positive expression rate of P53, C-myc and P16 were detected by immunocytochemical staining.

RESULTS

Quercetin at concentrations ranging from 40 mumol/L to 100 mumol/L significantly inhibited the proliferation of MGC-803 cells in a dose- and time-dependent manner (P<0.01). TUNEL assay indicated that the number of apoptotic cells in quercetin-treated group was greater than that in the control group (P<0.01). Expression of P53 and C-myc protein decreased following quercetin induction in a dose-dependent manner, whereas P16 expression increased significantly compared with that of the control group (P<0.01).

CONCLUSION

Quercetin can inhibit the growth and induce apoptosis of MGC-803 cells in a dose- and time-dependent manner. Its mechanisms may be relevant to the down-regulation of P53 and C-myc protein expression as well as up-regulation of P16 expression.

摘要

目的

研究槲皮素对人胃癌细胞系MGC - 803生长和凋亡的影响。

方法

采用MTT法和TUNEL法检测槲皮素的抑制率和凋亡指数（AI）。通过免疫细胞化学染色检测P53、C - myc和P16的阳性表达率。

结果

40μmol/L至100μmol/L浓度的槲皮素能显著抑制MGC - 803细胞的增殖，呈剂量和时间依赖性（P<0.01）。TUNEL检测表明，槲皮素处理组的凋亡细胞数多于对照组（P<0.01）。槲皮素诱导后，P53和C - myc蛋白表达呈剂量依赖性降低，而P16表达与对照组相比显著增加（P<0.01）。