Chen Xue, Wang Lan-lan, Cai Bei, Chen Jie, Feng Wei-hua
Clinical Immunological Laboratory, Department of Laboratory Medicine, Huaxi Hospital, Sichuan University, Chengdu 610041, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Sep;22(5):588-90.
To explore the relationship between Fas-FasL-mediated signal transduction pathway and apoptosis disorder of T lymphocyte subset in SLE patients.
The expression rate of membrane Fas, FasL and that of intracellular activated caspase-3 of T lymphocyte subset were determined by flow cytometry.
Compared with healthy control group, the expression rate of membrane Fas on CD4(+) T cells significantly increased in SLE patients in the active and inactive phases (P<0.05), however, that on CD8(+) T cells slightly increased but there was no statistical significance (P>0.05). The expression rate of FasL on T cell subset in SLE patients in the active and inactive phases significantly increased (P<0.05) but there was no obvious difference of the expression rate of Fas and FasL on T cell subset between the two disease groups (P>0.05). The expression rate of intracellular activated caspase-3 in T cell subset of SLE patients in the active phase was notably higher than that in the inactive phase and healthy control group (P<0.05). The expression rate of intracellular activated caspase-3 in T cell subset of SLE patients in the inactive phase was slightly higher than that in health control group but there was no statistical significance.
Apoptotic speed of T lymphocyte subset in SLE patients was accelerated while CD4(+) T cells were in a state of active apoptosis. It is possible that Fas-FasL signal transduction pathway plays an important role in the induction of T cell apoptosis. The degree of apoptosis of T lymphocytes closely correlates with the disease's activity in SLE patients.
探讨Fas - FasL介导的信号转导通路与系统性红斑狼疮（SLE）患者T淋巴细胞亚群凋亡紊乱之间的关系。
采用流式细胞术检测T淋巴细胞亚群膜Fas、FasL的表达率及细胞内活化的半胱天冬酶 - 3的表达率。
与健康对照组相比，SLE患者活动期和非活动期CD4(+) T细胞上膜Fas的表达率显著升高（P<0.05），而CD8(+) T细胞上膜Fas的表达率略有升高但无统计学意义（P>0.05）。SLE患者活动期和非活动期T细胞亚群上FasL的表达率显著升高（P<0.05），但两个疾病组之间T细胞亚群上Fas和FasL表达率无明显差异（P>0.05）。SLE患者活动期T细胞亚群内活化的半胱天冬酶 - 3的表达率显著高于非活动期和健康对照组（P<0.05）。SLE患者非活动期T细胞亚群内活化的半胱天冬酶 - 3的表达率略高于健康对照组，但无统计学意义。
SLE患者T淋巴细胞亚群凋亡速度加快，而CD4(+) T细胞处于主动凋亡状态。Fas - FasL信号转导通路可能在诱导T细胞凋亡中起重要作用。SLE患者T淋巴细胞凋亡程度与疾病活动度密切相关。
