Zhong Yong-Gen, Feng Wei-Ying, Luo Hong-Qiang, Fu Jia-Ping, Jin Jie
Department of Hematology, Shaoxing People's Hospital, Shaoxing 312000, China.
Zhonghua Xue Ye Xue Za Zhi. 2008 May;29(5):329-32.
To explore the relationship between Fas-FasL-mediated signal transduction pathway and apoptosis of T lymphocyte subset in ITP patients.
The expression rates of membrane Fas, FasL and intracellular activated caspase-3 in peripheral T lymphocyte subset were determined by flow cytometry. T cell subsets with caspase-3 protein expression were detected by Western blot.
As compared with that in healthy control group [(29.4 +/- 8.2)%], the expression rate of membrane Fas on CD4+ T cells was significantly increased in ITP patients [(42.1 +/- 9.5)%] (P < 0.05), however, that on CD8+ T cells was only slightly increased [(9.3 +/- 6.0)% vs (13.4 +/- 5.8)%] with no statistical significance (P > 0.05). The expression rate of FasL on T cell subset in ITP patients was significantly increased (P < 0.05), and that of intracellular activated caspase-3 in T cell subset in ITP patients was notably higher than that in healthy control group (P < 0.05). Western blot analysis showed that the expression of pro-caspase-3 and cleaved-caspase-3 in CD4+ T cells in patients with ITP after treatment were significantly reduced compared with those before treatment (P < 0.05).
Apoptosis of T lymphocyte subset in ITP patients is accelerated. It is possible that Fas-FasL signal transduction pathway plays an important role in the induction of the apoptosis. The degree of apoptosis of T lymphocytes closely correlates with the disease's activity in ITP patients. Hormone therapy may interfere with Fas-FasL signal transduction pathway of apoptosis.
探讨特发性血小板减少性紫癜（ITP）患者中Fas-FasL介导的信号转导通路与T淋巴细胞亚群凋亡之间的关系。
采用流式细胞术检测外周血T淋巴细胞亚群中膜Fas、FasL及细胞内活化的半胱天冬酶-3的表达率。用蛋白质印迹法检测有半胱天冬酶-3蛋白表达的T细胞亚群。
与健康对照组[（29.4±8.2）%]相比，ITP患者CD4⁺T细胞上膜Fas的表达率显著升高[（42.1±9.5）%]（P<0.05），而CD8⁺T细胞上膜Fas的表达率仅略有升高[（9.3±6.0）%对（13.4±5.8）%]，无统计学意义（P>0.05）。ITP患者T细胞亚群上FasL的表达率显著升高（P<0.05），且ITP患者T细胞亚群中细胞内活化的半胱天冬酶-3的表达率明显高于健康对照组（P<0.05）。蛋白质印迹分析显示，ITP患者治疗后CD4⁺T细胞中前半胱天冬酶-3和裂解的半胱天冬酶-3的表达与治疗前相比显著降低（P<0.05）。
ITP患者T淋巴细胞亚群凋亡加速。Fas-FasL信号转导通路可能在诱导凋亡中起重要作用。ITP患者T淋巴细胞凋亡程度与疾病活动度密切相关。激素治疗可能干扰凋亡的Fas-FasL信号转导通路。
