Miyashita Tomoya, Yamaguchi Tokio, Motoyama Keiko, Unno Keiko, Nakano Yumiko, Shimoi Kayoko
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
Biochem Biophys Res Commun. 2006 Oct 20;349(2):775-80. doi: 10.1016/j.bbrc.2006.08.098. Epub 2006 Aug 24.
We investigated whether or not three kinds of social stress (isolation, crowding, and confrontation) increase urinary excretion of biopyrrins, oxidative metabolites of bilirubin, in mice. Male BALB/c mice (4 weeks old) were housed 5 per cage for 10 days. After acclimatization, mice were exposed to the above mentioned three kinds of psychosocial stress. Mouse blood and urine samples were collected after 2, 7, and 30 days of stress. Serum levels of corticosterone and urinary levels of biopyrrins were determined by EIA and ELISA, respectively. Adrenal hypertrophy, significant increases in serum concentration of corticosterone and in urinary excretion of biopyrrins were observed in mice exposed to these types of social stress for 7 days. These levels decreased after 30 days, though they were still rather high compared to the control group. These results suggested that social stress causes oxidative stress and that biopyrrins could be useful biomarkers of psychosocial stress.
我们研究了三种社会压力（隔离、拥挤和对抗）是否会增加小鼠尿液中胆红素氧化代谢产物——生物吡咯的排泄量。将雄性BALB/c小鼠（4周龄）每笼饲养5只，共饲养10天。适应环境后，使小鼠暴露于上述三种心理社会压力下。在施加压力2天、7天和30天后采集小鼠血液和尿液样本。分别通过酶免疫分析（EIA）和酶联免疫吸附测定（ELISA）测定血清皮质酮水平和尿液中生物吡咯水平。在暴露于这些类型的社会压力7天的小鼠中，观察到肾上腺肥大、血清皮质酮浓度显著升高以及生物吡咯尿排泄量增加。30天后这些水平下降，尽管与对照组相比仍相当高。这些结果表明，社会压力会导致氧化应激，并且生物吡咯可能是心理社会压力的有用生物标志物。
