Shashkov Alexander S, Kocharova Nina A, Zatonsky George V, Błaszczyk Aleksandra, Knirel Yuriy A, Rozalski Antoni
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Carbohydr Res. 2007 Feb 26;342(3-4):653-8. doi: 10.1016/j.carres.2006.08.005. Epub 2006 Sep 1.
The O-polysaccharide chain of the lipopolysaccharide (LPS) of Providencia stuartii O20 was found to contain d-glucuronic acid, N-acetyl-d-glucosamine, and a rarely occurring higher sugar 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyl-8-epilegionaminic acid, 8eLeg5Ac7Ac). Degradation of the LPS with dilute acetic acid caused depolymerization of the polysaccharide chain by the ketosidic linkage to give a tetrasaccharide corresponding to the repeating unit of the polysaccharide. Based on sugar and methylation analyses of the tetrasaccharide and O-deacylated LPS as well as ESIMS, (1)H and (13)C NMR spectroscopy data, the structure of the O-polysaccharide of P. stuartii O20 was established.
斯氏普罗威登斯菌O20脂多糖（LPS）的O-多糖链被发现含有D-葡萄糖醛酸、N-乙酰-D-葡萄糖胺，以及一种罕见的高级糖5,7-二乙酰氨基-3,5,7,9-四脱氧-L-甘油-D-半乳糖壬-2-酮糖酸（二-N-乙酰-8-表军团氨糖酸，8eLeg5Ac7Ac）。用稀乙酸降解LPS会导致多糖链通过酮糖苷键解聚，生成一种对应于多糖重复单元的四糖。基于对该四糖和O-脱酰基LPS的糖和甲基化分析以及电喷雾离子化质谱（ESIMS）、氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）光谱数据，确定了斯氏普罗威登斯菌O20的O-多糖结构。
