日本海栖热袍菌 KMM 3601 0-特异性多糖的结构，含有 5,7-二乙酰氨基-3,5,7,9-四脱氧-D-甘油-D-塔罗-壬-2-酮酸。

Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid.

机构信息

Paciﬁc Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.

出版信息

Biochemistry (Mosc). 2011 Jul;76(7):791-6. doi: 10.1134/S0006297911070091.

PMID:21999540

Abstract

Structure of the O-specific polysaccharide chain of the lipopolysaccharide (LPS) of Shewanella japonica KMM 3601 was elucidated. The initial and O-deacylated LPS as well as a trisaccharide representing the O-deacetylated repeating unit of the O-specific polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The polysaccharide was found to contain a rare higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid (a derivative of 4-epilegionaminic acid, 4eLeg). The following structure of the trisaccharide repeating unit was established: →4)-α-4eLegp5Ac7Ac-(2→4)-β-D-GlcpA3Ac-(1→3)-β-D-GalpNAc-(1→.

摘要

日本海栖热袍菌 KMM 3601 的脂多糖 (LPS) 的 O-特异性多糖链结构已被阐明。通过糖分析以及 1H 和 13C NMR 光谱研究了初始 LPS 和 O-去酰化 LPS 以及三糖代表 O-去乙酰化重复单元的 O-特异性多糖。发现多糖含有一种罕见的更高糖，即 5,7-二乙酰氨基-3,5,7,9-四脱氧-D-甘油-D-塔罗-壬-2-烯酸（4-表莱格氨基糖酸的衍生物，4eLeg）。三糖重复单元的结构如下：→4）-α-4eLegp5Ac7Ac-（2→4）-β-D-GlcpA3Ac-（1→3）-β-D-GalpNAc-（1→。