Ongvarrasopone Chalermporn, Roshorm Yaowaluck, Somyong Suthasinee, Pothiratana Chetsada, Petchdee Soontaree, Tangkhabuanbutra Jarasporn, Sophasan Samaisukh, Panyim Sakol
Institute of Molecular Biology and Genetics, Mahidol University (Salaya Campus), 25/25 Phutthamonthon 4 Rd. Salaya, Phutthamonthon district, Nakhon Pathom, 73170, Thailand.
Biochim Biophys Acta. 2006 Jul;1759(7):328-39. doi: 10.1016/j.bbaexp.2006.07.004. Epub 2006 Jul 29.
Serotonin (5-HT) mediates a number of diverse physiological functions in crustaceans by interacting with various 5-HT receptor subtypes. A putative 5-HT receptor cloned from the ovary of the black tiger prawn (Penaeus monodon) consisted of 2291 nucleotides, encoding a putative 5-HT(1Pem) receptor protein of 591 amino acids. Transient expression of 5-HT(1Pem) in HEK293 cells demonstrated a saturable [3H]-5-HT binding with a Kd of 10.43+/-1.13 nM and Bmax of 1.53+/-0.06 pmol/mg. The putative 5-HT(1Pem) receptor is expressed in all tissues examined and is constitutively expressed in the ovary during ovarian maturation and spent phase. Polyclonal antibodies against the third intracellular loop (i3 loop) of the 5-HT receptor showed that the 5-HT(1Pem) receptor protein was expressed in the trabeculae of ovarian stages 1 and 2 but on the cortical rod and surrounding the oocyte membrane of stages 3 and 4, suggesting that receptor localization plays a critical role in regulating ovarian maturation and spawning in penaeus shrimp.
血清素（5-羟色胺，5-HT）通过与多种5-HT受体亚型相互作用，介导甲壳类动物的多种生理功能。从黑虎虾（斑节对虾）卵巢中克隆出的一种假定的5-HT受体由2291个核苷酸组成，编码一种含591个氨基酸的假定的5-HT(1Pem)受体蛋白。5-HT(1Pem)在HEK293细胞中的瞬时表达显示出可饱和的[3H]-5-HT结合，解离常数（Kd）为10.43±1.13 nM，最大结合量（Bmax）为1.53±0.06 pmol/mg。这种假定的5-HT(1Pem)受体在所检测的所有组织中均有表达，并且在卵巢成熟和排卵后期的卵巢中持续表达。针对5-HT受体第三细胞内环（i3环）的多克隆抗体表明，5-HT(1Pem)受体蛋白在卵巢1期和2期的小梁中表达，但在3期和4期的皮质杆以及卵母细胞膜周围表达，这表明受体定位在调控对虾卵巢成熟和产卵中起关键作用。
