用于人鼻中隔软骨组织工程的人血清。
Human serum for tissue engineering of human nasal septal cartilage.
作者信息
Alexander Thomas H, Sage August B, Schumacher Barbara L, Sah Robert L, Watson Deborah
机构信息
Division of Otolaryngology-Head and Neck Surgery, University of California-San Diego, 200 W. Arbor Drive, San Diego, CA 92103, USA.
出版信息
Otolaryngol Head Neck Surg. 2006 Sep;135(3):397-403. doi: 10.1016/j.otohns.2006.05.029.
OBJECTIVE
To compare the chondrogenic and proliferative effects of pooled human serum (HS) and fetal bovine serum (FBS) on tissue-engineered human nasal septal chondrocytes.
STUDY DESIGN AND SETTING
Human chondrocytes were expanded for one passage in monolayer in medium supplemented with 10% FBS, 2% HS, 10% HS, or 20% HS. Cells were then suspended in alginate beads for 3D culture for 2 weeks with 10% FBS, 2% HS, 10% HS, or 20% HS.
RESULTS
Monolayer cell yields were greater with HS than FBS. In alginate, cellular proliferation, glycosaminoglycan production per cell, and type II collagen were significantly higher with 10% HS compared to 10% FBS controls.
CONCLUSION
HS results in increased proliferation and production of cartilaginous extracellular matrix by tissue-engineered human nasal septal chondrocytes, compared to FBS controls.
SIGNIFICANCE
Culture with human serum may facilitate creation of neocartilage constructs that more closely resemble native tissue.
目的
比较人混合血清（HS）和胎牛血清（FBS）对组织工程化人鼻中隔软骨细胞的软骨生成和增殖作用。
研究设计与设置
人软骨细胞在补充有10% FBS、2% HS、10% HS或20% HS的培养基中单层扩增一代。然后将细胞悬浮于藻酸盐珠中，在含有10% FBS、2% HS、10% HS或20% HS的条件下进行三维培养2周。
结果
HS培养的单层细胞产量高于FBS。在藻酸盐中，与10% FBS对照组相比，10% HS组的细胞增殖、每细胞糖胺聚糖产量和II型胶原蛋白含量显著更高。
结论
与FBS对照组相比，HS可使组织工程化人鼻中隔软骨细胞的增殖增加，并促进软骨细胞外基质的产生。
意义
用人血清培养可能有助于创建更接近天然组织的新软骨构建体。
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