骨形态发生蛋白2和7对藻酸盐中鼻中隔软骨细胞的作用。

Effect of bone morphogenetic proteins 2 and 7 on septal chondrocytes in alginate.

作者信息

Hicks David L, Sage August B, Shelton Elliot, Schumacher Barbara L, Sah Robert L, Watson Deborah

机构信息

Division of Head and Neck Surgery, University of California, San Diego, CA, USA.

出版信息

Otolaryngol Head Neck Surg. 2007 Mar;136(3):373-9. doi: 10.1016/j.otohns.2006.10.040.

DOI:10.1016/j.otohns.2006.10.040

PMID:17321862

Abstract

OBJECTIVE

To determine the effects of bone morphogenetic proteins (BMP)-2 and -7, and serum, on extracellular matrix production by human septal chondrocytes in alginate.

STUDY DESIGN

Human nasal septal chondrocytes were expanded, suspended in alginate, and cultured in BMP-2 or 7, with and without serum. The optimal concentration of each growth factor was determined based on matrix production. Next, the synergistic effects of BMP-2 and -7 at optimal concentrations were determined on separate beads, based on matrix quantity and histology.

RESULTS

Matrix content was highest with concentrations of BMP-2 and -7 of 100 ng/ml and 20 ng/ml, respectively, with serum. Adding both BMP-2 and -7, with serum, increased matrix content by factors of 5.1 versus serum-only cultures, 2.7 versus only BMP-2 with serum, and 2.4 versus only BMP-7 with serum. All comparisons were statistically significant.

CONCLUSION

BMP-2 and -7 significantly increase production of extracellular matrix by septal chondrocytes suspended in alginate. The presence of serum improves matrix production.

SIGNIFICANCE

BMP-2 and -7 have great potential for use in cartilage tissue engineering.

摘要

目的

确定骨形态发生蛋白（BMP）-2和-7以及血清对藻酸盐中人类鼻中隔软骨细胞产生细胞外基质的影响。

研究设计

将人类鼻中隔软骨细胞进行扩增，悬浮于藻酸盐中，并在添加或不添加血清的情况下，用BMP-2或BMP-7进行培养。根据基质产生情况确定每种生长因子的最佳浓度。接下来，基于基质数量和组织学，在单独的珠子上确定最佳浓度的BMP-2和BMP-7的协同作用。

结果

在添加血清的情况下，BMP-2浓度为100 ng/ml、BMP-7浓度为20 ng/ml时，基质含量最高。同时添加BMP-2和BMP-7并添加血清时，与仅添加血清的培养物相比，基质含量增加了5.1倍；与仅添加BMP-2并添加血清的培养物相比，增加了2.7倍；与仅添加BMP-7并添加血清的培养物相比，增加了2.4倍。所有比较均具有统计学意义。