Xu Chun-jiao, Guo Feng, Gao Qing-ping, Wu Ying-fang, Jian Xin-chun, Peng Jie-ying
Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2006 Aug;31(4):512-7.
To explore the effect of astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) scaffolds and bone marrow stem cells (BMSCs) on periodontal regeneration of experimentally horizontal periodontal defects in dogs.
Dog BMSCs were isolated from the bone marrow and then cultured in a conditioned medium to be induced for osteogenesis. The expressions of Type I collagen and alkaline phosphatase (ALP) were examined by immunohistochemistry and histochemistry in the induced BMSCs, respectively. The BMSCs were harvested and implanted with astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and chitosan/polylactic acid (C/PLA) scaffolds. Horizontal alveolar bone defects (5 mm depth, 2 mm width) were produced surgically in the buccal side of the mandibular premolar 3 and 4 of the 10 dogs. The defects were randomly repaired with a cell-scaffold construction (10 teeth per group): root planning only (surgical control), AP-C/PLA with a conditioned medium (medium control), C/PLA with BMSCs (scaffolds control), and AP-C/PLA with BMSCs (experimental group) . The dogs were killed at 4 weeks and 8 weeks after the surgery, and block sections of the defects were collected for the histologic and histometric analysis.
BMSCs induced in vitro exhibited an osteogenic phenotype with expressing Type I collagen and ALP histologically. The bone nodule structure was observed in the experimental group 4 weeks postsurgically. The engineered bone became more mature,similar to the native bone 8 weeks postsurgically. The amount of new bone regeneration and the rate of new bone filling to the defect height of the experimental group were significantly different from those of the surgical control, medium control, and scaffolds control [(2.90+/-0.41) mm vs (0.83+/-0.30) mm, (1.46+/-0.55) mm, (2.67+/-0.26) mm; 57.46% vs 15.68 %, 30.13%, 51.87%)] (P<0.01, P<0.01, P<0.05).
Astragalus polysaccharides can promote the new bone formation on the periodontal defects. The technology of tissue engineering with AP-C/PLA scaffolds and induced BMSCs may contribute to the periodontal regeneration.
探讨黄芪多糖-壳聚糖/聚乳酸（AP-C/PLA）支架与骨髓干细胞（BMSCs）对犬实验性水平牙周缺损牙周组织再生的影响。
从犬骨髓中分离出BMSCs，然后在条件培养基中培养以诱导成骨。分别通过免疫组织化学和组织化学检测诱导后的BMSCs中I型胶原蛋白和碱性磷酸酶（ALP）的表达。收集BMSCs，并与黄芪多糖-壳聚糖/聚乳酸（AP-C/PLA）和壳聚糖/聚乳酸（C/PLA）支架一起植入。对10只犬的下颌第3和第4前磨牙颊侧进行手术制造水平牙槽骨缺损（深度5mm，宽度2mm）。缺损随机采用细胞-支架构建物修复（每组10颗牙）：仅进行根面平整（手术对照组）、AP-C/PLA与条件培养基（培养基对照组）、C/PLA与BMSCs（支架对照组）以及AP-C/PLA与BMSCs（实验组）。术后4周和8周处死犬，收集缺损部位的块状切片进行组织学和组织计量学分析。
体外诱导的BMSCs在组织学上表现出表达I型胶原蛋白和ALP的成骨表型。术后4周实验组观察到骨结节结构。术后8周，构建的骨组织变得更加成熟，类似于天然骨。实验组新骨再生量及新骨填充至缺损高度的比例与手术对照组、培养基对照组和支架对照组相比有显著差异[（2.90±0.41）mm对（0.83±0.30）mm、（1.46±0.55）mm、（2.67±0.26）mm；57.46%对15.68%、30.13%、51.87%]（P<0.01，P<0.01，P<0.05）。
黄芪多糖可促进牙周缺损处新骨形成。AP-C/PLA支架与诱导后的BMSCs的组织工程技术可能有助于牙周组织再生。
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