Xu Chun-Jiao, Jian Xin-Chun, Peng Jie-Ying, Guo Feng, Huang Bai-Ying, Xiong Cheng-Dong, Pan Gao-Feng
Department of Periodontology; Xiangya Hospital, Central South University, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Jun;30(3):283-7.
To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.
BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.
Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.
AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
观察犬骨髓基质细胞（BMSCs）在黄芪多糖-壳聚糖/聚乳酸（AP-C/PLA）及壳聚糖/聚乳酸（C/PLA）材料上体外培养的生物学行为，寻找适合牙周组织工程的复合材料。
将BMSCs（用50mg/L维生素C、10⁻⁸mol/L地塞米松、10mmol/Lβ-甘油磷酸钠诱导14天）分别接种于AP-C/PLA或C/PLA材料上培养5天。用扫描电子显微镜观察BMSCs的黏附及形态，并计算结合率。采用免疫组织化学染色检测Ⅰ型胶原合成，用放射免疫法测定骨钙素含量。
BMSCs在AP-C/PLA上的结合率、Ⅰ型胶原合成及骨钙素含量均显著高于在C/PLA上。
AP-C/PLA可促进BMSCs的增殖、分化及细胞外基质合成，可作为骨组织工程良好的支架材料。
