Previte Michael J R, Aslan Kadir, Malyn Stuart, Geddes Chris D
Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, Institute of Fluorescence, University of Maryland Biotechnology Institute, 725 West Lombard St., Baltimore, MD 21201, USA.
J Fluoresc. 2006 Sep;16(5):641-7. doi: 10.1007/s10895-006-0121-9. Epub 2006 Sep 2.
In this rapid communication we describe a new approach to protein detection with chemiluminescence. By combining common practices in protein detection with chemiluminescence, microwave technology, and metal-enhanced chemiluminescence, we show that we can use low power microwaves to substantially increase enzymatic chemiluminescent reaction rates on metal substrates. As a result, we have found that we can in essence trigger chemiluminescence with low power microwave (Mw) pulses and ultimately, perform on-demand protein detection assays. Using microwave triggered metal-enhanced chemiluminescence (MT-MEC), we not only improve the sensitivity of immunoassays with enhanced signal-to-noise ratios, but we also show that we can accurately quantify protein concentrations by integrating the photon flux for discrete time intervals.
在本快报中，我们描述了一种利用化学发光进行蛋白质检测的新方法。通过将蛋白质检测的常规方法与化学发光、微波技术和金属增强化学发光相结合，我们表明可以使用低功率微波大幅提高金属底物上的酶促化学发光反应速率。结果，我们发现本质上可以用低功率微波（Mw）脉冲触发化学发光，并最终进行按需蛋白质检测分析。利用微波触发的金属增强化学发光（MT-MEC），我们不仅通过提高信噪比提高了免疫分析的灵敏度，还表明可以通过对离散时间间隔内的光子通量进行积分来准确量化蛋白质浓度。
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