Parfenov Alexandr, Gryczynski Ignacy, Malicka Joanna, Geddes Chris D, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201.
J Phys Chem B. 2003;107(34):8829-8833. doi: 10.1021/jp022660r.
Recent reports have shown enhanced fluorescence for fluorophores in close proximity to chemically deposited silver islands or colloids. To expand the usefulness of metal-enhanced fluorescence we tested fractal silver structures formed on, or near, silver electrodes by passage of electric currents. The emission intensity of fluorescein-labeled human serum albumin (FITC-HSA) was enhanced over 100-fold when adsorbed to the fractal silver structures as compared to glass. The amplitude-weighted lifetime is dramatically reduced to near 3 ps. Enhanced fluorescence was shown to result in selective observation of FITC-HSA over a fluorophore not attached to the silver surface. And finally, photostability measurements indicate 160-fold more photons are detectable from FITC-HSA on the fractal silver surface. These results suggest the use of in situ generated silver structures for metal-enhanced fluorescence.
最近的报告显示，靠近化学沉积银岛或胶体的荧光团荧光增强。为了扩大金属增强荧光的用途，我们测试了通过电流在银电极上或其附近形成的分形银结构。与玻璃相比，荧光素标记的人血清白蛋白（FITC-HSA）吸附到分形银结构上时，发射强度增强了100多倍。振幅加权寿命显著缩短至接近3皮秒。结果表明，增强荧光可用于选择性观察FITC-HSA，而不观察未附着在银表面的荧光团。最后，光稳定性测量表明，在分形银表面上，从FITC-HSA中可检测到的光子数量多出160倍。这些结果表明可使用原位生成的银结构实现金属增强荧光。
