Jacobs E, Röck R, Dalehite L
Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany.
Infect Immun. 1990 Aug;58(8):2464-9. doi: 10.1128/iai.58.8.2464-2469.1990.
The identification of specific T cell and B cell epitopes of the P1 protein, which functions as an adhesin and as a major antigen of Mycoplasma pneumoniae, has become of central interest for the design of synthetic vaccines. Here we report the isolation from guinea pigs infected intranasally with M. pneumoniae of hilar and bronchial T lymphocytes which proliferated after in vitro stimulation with sonicated M. pneumoniae whole-cell antigen and with the isolated P1 protein. For more detailed information on T cell epitopes, a 51-amino-acid region (histidine 821 to glycine 871; numbered from the NH2-terminal end) of the P1 protein was analyzed for a T cell epitope. An octapeptide, S-G-S-R-S-F-L-P (starting at amino acid 845), stimulated in vitro lymphocytes of bronchial washings and of hilar lymph nodes. Furthermore, this T cell-stimulating amino acid sequence was located at the C-terminal end of a B cell epitope with the sequence T-N-T (starting at amino acid 842).
P1蛋白作为肺炎支原体的黏附素和主要抗原，对其特定T细胞和B细胞表位的鉴定已成为合成疫苗设计的核心关注点。在此，我们报告从经鼻内感染肺炎支原体的豚鼠中分离出肺门和支气管T淋巴细胞，这些细胞在体外用超声处理的肺炎支原体全细胞抗原和分离出的P1蛋白刺激后发生增殖。为获取有关T细胞表位的更详细信息，对P1蛋白的一个51个氨基酸的区域（从NH2末端起编号的组氨酸821至甘氨酸871）进行了T细胞表位分析。一种八肽，S-G-S-R-S-F-L-P（从氨基酸845开始），可刺激支气管灌洗物和肺门淋巴结的体外淋巴细胞。此外，这个刺激T细胞的氨基酸序列位于一个序列为T-N-T（从氨基酸842开始）的B细胞表位的C末端。
