肺炎支原体黏附素的免疫显性表位
Immunodominant epitopes of the adhesin of Mycoplasma pneumoniae.
作者信息
Jacobs E, Pilatschek A, Gerstenecker B, Oberle K, Bredt W
机构信息
Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1194-7. doi: 10.1128/jcm.28.6.1194-1197.1990.
Abstract
Overlapping octapeptides from the amino acid sequence of the adherence protein of Mycoplasma pneumoniae were synthesized and used as the antigen in an enzyme-linked immunosorbent assay with serum samples from M. pneumoniae-infected patients. Of a sequence of 338 amino acids positioned between leucine 801 and leucine 1139, only two regions with immunodominant continuous epitopes were detected. The immunoglobulin G and immunoglobulin M antibodies of child and adult patients reacted especially with the NH2-(810)-W-I-G-N-G-Y-R-Y peptide but also reacted with the NH2-(1124)-F-T-D-F-V-K-P-R peptide.
摘要
合成了来自肺炎支原体黏附蛋白氨基酸序列的重叠八肽，并将其用作酶联免疫吸附测定中的抗原，检测肺炎支原体感染患者血清样本。在位于亮氨酸801和亮氨酸1139之间的338个氨基酸序列中，仅检测到两个具有免疫显性连续表位的区域。儿童和成年患者的免疫球蛋白G和免疫球蛋白M抗体尤其与NH2-(810)-W-I-G-N-G-Y-R-Y肽发生反应，但也与NH2-(1124)-F-T-D-F-V-K-P-R肽发生反应。
相似文献
1
Immunodominant epitopes of the adhesin of Mycoplasma pneumoniae.肺炎支原体黏附素的免疫显性表位
J Clin Microbiol. 1990 Jun;28(6):1194-7. doi: 10.1128/jcm.28.6.1194-1197.1990.
2
Molecular mimicry by Mycoplasma pneumoniae to evade the induction of adherence inhibiting antibodies.肺炎支原体通过分子模拟来逃避诱导黏附抑制抗体。
J Med Microbiol. 1995 Dec;43(6):422-9. doi: 10.1099/00222615-43-6-422.
3
Binding sites of attachment-inhibiting monoclonal antibodies and antibodies from patients on peptide fragments of the Mycoplasma pneumoniae adhesin.肺炎支原体粘附素肽片段上附着抑制性单克隆抗体及患者抗体的结合位点
Infect Immun. 1989 Mar;57(3):685-8. doi: 10.1128/iai.57.3.685-688.1989.
4
Topological mapping of the P1-adhesin of Mycoplasma pneumoniae with adherence-inhibiting monoclonal antibodies.
J Gen Microbiol. 1990 Mar;136(3):471-6. doi: 10.1099/00221287-136-3-471.
5
Identification of P1 gene domain containing epitope(s) mediating Mycoplasma pneumoniae cytoadherence.鉴定包含介导肺炎支原体细胞黏附的表位的P1基因结构域。
J Exp Med. 1988 Feb 1;167(2):718-23. doi: 10.1084/jem.167.2.718.
6
Human antibody response to the major adhesin of Mycoplasma pneumoniae: increase in titers against synthetic peptides in patients with pneumonia.
APMIS. 1991 Jun;99(6):515-20. doi: 10.1111/j.1699-0463.1991.tb05184.x.
7
Shared epitopes between Mycoplasma pneumoniae major adhesin protein P1 and a 140-kilodalton protein of Mycoplasma genitalium.肺炎支原体主要黏附蛋白P1与生殖支原体140千道尔顿蛋白之间的共同表位。
Infect Immun. 1987 Jan;55(1):49-56. doi: 10.1128/iai.55.1.49-56.1987.
8
Use of adherence protein of Mycoplasma pneumoniae as antigen for enzyme-linked immunosorbent assay (ELISA).使用肺炎支原体黏附蛋白作为酶联免疫吸附测定（ELISA）的抗原。
Isr J Med Sci. 1987 Jun;23(6):709-12.
9
A B cell-, T cell-linked epitope located on the adhesin of Mycoplasma pneumoniae.位于肺炎支原体黏附素上的一个B细胞和T细胞相关表位。
Infect Immun. 1990 Aug;58(8):2464-9. doi: 10.1128/iai.58.8.2464-2469.1990.
10
Indirect enzyme-linked immunosorbent assay for detection of immunoglobulin G reactive with a recombinant protein expressed from the gene encoding the 116-kilodalton protein of Mycoplasma pneumoniae.间接酶联免疫吸附测定法，用于检测与肺炎支原体116千道尔顿蛋白编码基因表达的重组蛋白发生反应的免疫球蛋白G。
J Clin Microbiol. 1999 Apr;37(4):1024-9. doi: 10.1128/JCM.37.4.1024-1029.1999.
引用本文的文献
1
Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae.来自人类病原体肺炎支原体的免疫优势蛋白 P1 和 P40/P90。
Nat Commun. 2020 Oct 14;11(1):5188. doi: 10.1038/s41467-020-18777-y.
2
Evaluating polymicrobial immune responses in patients suffering from tick-borne diseases.评估患有蜱传疾病患者的多微生物免疫反应。
Sci Rep. 2018 Oct 29;8(1):15932. doi: 10.1038/s41598-018-34393-9.
3
Analysis of the Mycoplasma genitalium MgpB Adhesin to Predict Membrane Topology, Investigate Antibody Accessibility, Characterize Amino Acid Diversity, and Identify Functional and Immunogenic Epitopes.解脲脲原体MgpB黏附素的分析，用于预测膜拓扑结构、研究抗体可及性、表征氨基酸多样性以及鉴定功能和免疫原性表位。
PLoS One. 2015 Sep 18;10(9):e0138244. doi: 10.1371/journal.pone.0138244. eCollection 2015.
4
Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection.纳米棒阵列表面增强拉曼光谱对肺炎支原体检测的特异性及菌株分型能力
PLoS One. 2015 Jun 29;10(6):e0131831. doi: 10.1371/journal.pone.0131831. eCollection 2015.
5
Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene.肺炎支原体P1基因免疫显性和细胞黏附区段的描绘
BMC Microbiol. 2014 Apr 28;14:108. doi: 10.1186/1471-2180-14-108.
6
Strategy to create chimeric proteins derived from functional adhesin regions of Mycoplasma pneumoniae for vaccine development.用于疫苗开发的源自肺炎支原体功能性粘附素区域的嵌合蛋白构建策略。
Infect Immun. 2009 Nov;77(11):5007-15. doi: 10.1128/IAI.00268-09. Epub 2009 Aug 10.
7
Expression and immunological characterization of the carboxy-terminal region of the P1 adhesin protein of Mycoplasma pneumoniae.肺炎支原体P1黏附蛋白羧基末端区域的表达及免疫学特性
J Clin Microbiol. 2005 Jan;43(1):321-5. doi: 10.1128/JCM.43.1.321-325.2005.
8
Mycoplasma pneumoniae and its role as a human pathogen.肺炎支原体及其作为人类病原体的作用。
Clin Microbiol Rev. 2004 Oct;17(4):697-728, table of contents. doi: 10.1128/CMR.17.4.697-728.2004.
9
Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains.个别菌株P1黏附素基因内肺炎支原体P1 1型和2型特异性序列。
Infect Immun. 2001 Sep;69(9):5612-8. doi: 10.1128/IAI.69.9.5612-5618.2001.
10
Identification of a new variable sequence in the P1 cytadhesin gene of Mycoplasma pneumoniae: evidence for the generation of antigenic variation by DNA recombination between repetitive sequences.肺炎支原体P1黏附素基因中一个新可变序列的鉴定：重复序列间DNA重组产生抗原变异的证据
Infect Immun. 1999 Sep;67(9):4557-62. doi: 10.1128/IAI.67.9.4557-4562.1999.
本文引用的文献
1
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
2
Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody.肺炎支原体黏附素通过单克隆抗体定位于顶端结构。
Nature. 1982 Aug 19;298(5876):765-7. doi: 10.1038/298765a0.
3
Molecular basis for cytadsorption of Mycoplasma pneumoniae.肺炎支原体细胞吸附的分子基础。
J Bacteriol. 1982 Sep;151(3):1514-22. doi: 10.1128/jb.151.3.1514-1522.1982.
4
Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle.肺炎支原体感染：一种表面蛋白在附着细胞器中的作用
Science. 1982 Apr 16;216(4543):313-5. doi: 10.1126/science.6801766.
5
Pneumonia: an eleven-year study in a pediatric practice.
Am J Epidemiol. 1981 Jan;113(1):12-21. doi: 10.1093/oxfordjournals.aje.a113061.
6
Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.利用肽合成技术探测病毒抗原的表位，分辨率可达单个氨基酸水平。
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3998-4002. doi: 10.1073/pnas.81.13.3998.
7
Prediction of protein antigenic determinants from amino acid sequences.从氨基酸序列预测蛋白质抗原决定簇。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8. doi: 10.1073/pnas.78.6.3824.
8
Tissue cultures and mycoplasmas.组织培养与支原体。
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+.
9
Antibodies in the sera of Mycoplasma pneumoniae-infected patients against proteins of Mycoplasma genitalium and other mycoplasmas of man.肺炎支原体感染患者血清中针对生殖支原体及其他人类支原体蛋白质的抗体。
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Aug;266(1-2):32-42. doi: 10.1016/s0176-6724(87)80018-4.
10
Isolation of the adherence protein of Mycoplasma pneumoniae by fractionated solubilization and size exclusion chromatography.通过分级溶解和尺寸排阻色谱法分离肺炎支原体的黏附蛋白。
Biol Chem Hoppe Seyler. 1988 Dec;369(12):1295-9. doi: 10.1515/bchm3.1988.369.2.1295.
Zotero 插件