Jacobs E, Pilatschek A, Gerstenecker B, Oberle K, Bredt W
Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany.
J Clin Microbiol. 1990 Jun;28(6):1194-7. doi: 10.1128/jcm.28.6.1194-1197.1990.
Overlapping octapeptides from the amino acid sequence of the adherence protein of Mycoplasma pneumoniae were synthesized and used as the antigen in an enzyme-linked immunosorbent assay with serum samples from M. pneumoniae-infected patients. Of a sequence of 338 amino acids positioned between leucine 801 and leucine 1139, only two regions with immunodominant continuous epitopes were detected. The immunoglobulin G and immunoglobulin M antibodies of child and adult patients reacted especially with the NH2-(810)-W-I-G-N-G-Y-R-Y peptide but also reacted with the NH2-(1124)-F-T-D-F-V-K-P-R peptide.
合成了来自肺炎支原体黏附蛋白氨基酸序列的重叠八肽,并将其用作酶联免疫吸附测定中的抗原,检测肺炎支原体感染患者血清样本。在位于亮氨酸801和亮氨酸1139之间的338个氨基酸序列中,仅检测到两个具有免疫显性连续表位的区域。儿童和成年患者的免疫球蛋白G和免疫球蛋白M抗体尤其与NH2-(810)-W-I-G-N-G-Y-R-Y肽发生反应,但也与NH2-(1124)-F-T-D-F-V-K-P-R肽发生反应。