Nagato Y, Shimai K, Kushida T, Kushida H
Department of Anatomy, Tokai University School of Medicine, Isehara, Japan.
J Electron Microsc (Tokyo). 1990;39(2):115-9.
For a correlative light and electron microscopy of intestinal goblet cells, postembedding staining with ruthenium red (RR) was performed in epoxy-embedded sections. Tissue blocks were fixed in buffered aldehyde and embedded in a mixture of Quetol 651, nonenyl succinic anhydride (NSA), methyl nadic anhydride (MNA), and DMP-30. Sections at 0.4-0.5 micron in thickness were mounted on grids and were treated with an aqueous solution of RR followed by osmium tetroxide, uranyl acetate and lead citrate. Postembedding staining of epoxy sections revealed the interaction between RR and anionic groups by both light and electron microscopy. Light and electron microscopic observation of identical sites in semithin sections was successful for the correlations of colored reaction with electron density.
为了对肠道杯状细胞进行相关的光镜和电镜观察，在用环氧树脂包埋的切片中进行了钌红（RR）后包埋染色。组织块用缓冲醛固定，然后包埋在Quetol 651、壬烯基琥珀酸酐（NSA）、甲基纳迪克酸酐（MNA）和DMP - 30的混合物中。将厚度为0.4 - 0.5微米的切片装载到载网上，先用RR水溶液处理，然后依次用四氧化锇、醋酸铀和柠檬酸铅处理。环氧树脂切片的后包埋染色通过光镜和电镜揭示了RR与阴离子基团之间的相互作用。在半薄切片中对相同部位进行光镜和电镜观察，成功实现了显色反应与电子密度的相关性研究。
