Linss W, Kiss A, Geyer G
Acta Histochem. 1979;64(2):206-12.
We suggest a method for the section staining of polyanions by means of ruthenium red and osmium tetroxide. A 5 min incubation in diluted ruthenium red-OsO4 solution pH = 7.4 of ultrathin Durcupan-sections previously treated with sodium methoxide results in the opaque stining of mast cell granules, cartilage proteoglycans, and various epithelial mucosubstances.
我们提出了一种利用钌红和四氧化锇对多阴离子进行切片染色的方法。将预先用甲醇钠处理过的超薄杜尔库潘切片在pH = 7.4的稀释钌红-四氧化锇溶液中孵育5分钟,可使肥大细胞颗粒、软骨蛋白聚糖和各种上皮粘膜物质产生不透明染色。