Zhang Huige, Tian Kan, Tang Jianghong, Qi Shengda, Chen Hongli, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou, China.
J Chromatogr A. 2006 Oct 6;1129(2):304-7. doi: 10.1016/j.chroma.2006.08.016. Epub 2006 Sep 6.
Microemulsion electrokinetic chromatography (MEEKC) using 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM-BF4) ionic liquid (IL) as additive was developed for the analysis of baicalin, wogonin and baicalein in Scutellariae radix and its preparation. After conducting a series of optimizations, baseline separation was obtained for the analytes within 5min under the optimum conditions (sodium dodecyl sulfate (SDS) 0.88% (m/v) ethyl acetate 0.8% (v/v) butan-1-ol 0.2% (v/v) and the buffer composition were 25% acetonitrile (v/v), 7.5 mM BMIM-BF4 and 10 mM NaH2PO4, pH 8.2, applied voltage 17.5 kV and detection at 254 nm), the method has been successfully applied to the determination and quantification of the analytes in the extracts of S. radix (cooked), S. radix (raw) and Qingfeiyihuowan which was the preparation including S. radix.
以1-丁基-3-甲基咪唑四氟硼酸盐（BMIM-BF4）离子液体（IL）为添加剂的微乳液电动色谱法（MEEKC）用于分析黄芩及其制剂中的黄芩苷、汉黄芩素和黄芩苷元。经过一系列优化，在最佳条件下（十二烷基硫酸钠（SDS）0.88%（m/v）、乙酸乙酯0.8%（v/v）、丁醇-1 0.2%（v/v），缓冲液组成为25%乙腈（v/v）、7.5 mM BMIM-BF4和10 mM NaH2PO4，pH 8.2，施加电压17.5 kV，检测波长254 nm），5分钟内各分析物实现了基线分离。该方法已成功应用于熟黄芩、生黄芩提取物以及含黄芩制剂清肺抑火丸中各分析物的测定和定量分析。
