[栽培黄芩与野生黄芩化学成分的比较研究]

[Comparative study on chemcial composition between cultivated and wild Scutellariae radix].

作者信息

Li Wei, Li Hua, Yang Bin, Feng Xue-feng, Ge Xiao-guang, Liu Jin-yun

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(12):1425-9.

PMID:18837348

Abstract

OBJECTIVE

To do some comparative study on chemical composition between the cultivated and wild samples of Radix Scutellariae.

METHOD

Thirty three samples of Radix Scutellariae were collected from different habitats. An HPLC method for fingerprint identification and simultaneous determination of baicalin, baicalein and wogonin was established, and the content of ethanolic extractive was measured according to the method in Chinese Pharmacopoeia.

RESULT

The number of chromatographic peaks in fingerprint of wild sample was more than that of cultivated sample in Hebei and Inner Mongolia. In 95% confidence intervals, the contents of baicalin, baicalein and wogonin were (15.89 +/- 1.52)%, (1.04 +/- 0.26)% and (0.27 +/- 0.07)%, respectively in the cultivated samples and (11.93 +/- 1.62)%, (1.03 +/- 0.26)% and (0.27 +/- 0.06)%, respectively in wild samples. The content of ethanolic extractive was 52.07 +/- 3.05% in the cultivated and (41.21 +/- 2.86)% in the wild within 95% confidence interval.

CONCLUSION

Slight difference between the fingerprint of the cultivated and wild samples was found, and the contents of baicalin and ethanolic extraction in the cultivated were higher than those in the wild.

摘要

目的

对栽培黄芩与野生黄芩的化学成分进行比较研究。

方法

从不同产地采集33份黄芩样品。建立了用于指纹图谱鉴定及同时测定黄芩苷、黄芩素和汉黄芩素的高效液相色谱法，并按照《中国药典》方法测定乙醇提取物含量。

结果

河北和内蒙古的野生黄芩样品指纹图谱中的色谱峰数量多于栽培黄芩样品。在95%置信区间内，栽培黄芩样品中黄芩苷、黄芩素和汉黄芩素的含量分别为(15.89±1.52)%、(1.04±0.26)%和(0.27±0.07)%，野生黄芩样品中分别为(11.93±1.62)%、(1.03±0.26)%和(0.27±0.06)%。95%置信区间内，栽培黄芩乙醇提取物含量为52.07±3.05%，野生黄芩为(41.21±2.86)%。