Ilyas A A, Chou D K, Jungalwala F B, Costello C, Quarles R H
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
J Neurochem. 1990 Aug;55(2):594-601. doi: 10.1111/j.1471-4159.1990.tb04175.x.
A high proportion of patients with neuropathy have immunoglobulin M (IgM) paraproteins that react with carbohydrate determinants on the myelin-associated glycoprotein (MAG) and two sphingoglycolipids, 3-sulfoglucuronyl paragloboside (SGPG) and 3-sulfoglucuronyl lactosaminyl paragloboside. In order to characterize the fine specificities of these human antibodies further, the binding of 10 anti-MAG paraproteins to several chemically modified derivatives of SGPG was compared with the binding to intact SGPG by both TLC-overlay and enzyme-linked immunosorbent assay. The following derivatives were tested: the desulfated lipid, glucuronyl paragloboside (GPG); the methyl ester of GPG (MeGPG); the methyl ester of SGPG, 3-sulfomethylglucuronyl paragloboside (SMeGPG); and 3-sulfoglucosyl paragloboside (SGlcPG) produced by reduction of the carboxyl group of the glucuronic acid with sodium borohydride. All 10 IgM paraproteins and the related mouse IgM antibody, HNK-1, reacted most strongly with intact SGPG, but variations in the reactivity with the derivatives revealed striking differences in the structural requirements for binding between the antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)
很大比例的神经病变患者存在免疫球蛋白M（IgM）副蛋白，其可与髓鞘相关糖蛋白（MAG）以及两种鞘糖脂（3-磺基葡糖醛酰副球蛋白（SGPG）和3-磺基葡糖醛酰乳糖胺基副球蛋白）上的碳水化合物决定簇发生反应。为了进一步明确这些人源抗体的精细特异性，通过薄层层析覆盖法和酶联免疫吸附测定法，比较了10种抗MAG副蛋白与SGPG的几种化学修饰衍生物的结合情况以及与完整SGPG的结合情况。测试了以下衍生物：脱硫酸脂质、葡糖醛酰副球蛋白（GPG）；GPG的甲酯（MeGPG）；SGPG的甲酯、3-磺甲基葡糖醛酰副球蛋白（SMeGPG）；以及用硼氢化钠还原葡糖醛酸羧基生成的3-磺基葡糖基副球蛋白（SGlcPG）。所有10种IgM副蛋白以及相关的小鼠IgM抗体HNK-1与完整SGPG反应最强，但与衍生物反应性的差异揭示了抗体之间结合的结构要求存在显著差异。（摘要截短于250字）
