Chen Jyh-Ping, Lin Chia-Tseng
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, ROC.
J Biosci Bioeng. 2006 Jul;102(1):41-5. doi: 10.1263/jbb.102.41.
A galactose moiety was introduced into the fiber surface of a vegetable sponge by the covalent binding of lactobionic acid. The galactosylated sponge was used as scaffold for the culture of rat hepatocytes in a packed-bed bioreactor. Hepatocytes could be dynamically seeded into and uniformly distributed throughout the scaffold, and the immobilized cells maintained high albumin and urea production rates during long-term perfusion culture. The hepatocytes showed an increasing albumin production rate from 49 to 109 microg/10(6) cells/d over the 7-d culture.
通过乳糖酸的共价结合将半乳糖部分引入植物海绵的纤维表面。半乳糖基化海绵用作填充床生物反应器中大鼠肝细胞培养的支架。肝细胞可以动态接种到支架中并均匀分布在整个支架中，并且固定化细胞在长期灌注培养期间保持高白蛋白和尿素产生率。在7天的培养过程中，肝细胞的白蛋白产生率从49微克/10(6)个细胞/天增加到109微克/10(6)个细胞/天。
