Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi, 110042, India.
Regen Med. 2024;19(7-8):421-437. doi: 10.1080/17460751.2024.2378615. Epub 2024 Aug 5.
The paper highlights how significant characteristics of liver can be modeled in tissue-engineered constructs using unconventional scaffolds. Hepatic lobular organization and metabolic zonation can be mimicked with decellularized plant structures with vasculature resembling a native-hepatic lobule vascular arrangement or silk blend scaffolds meticulously designed for guided cellular arrangement as hepatic patches or metabolic activities. The functionality of hepatocytes can be enhanced and maintained for long periods in naturally fibrous structures paving way for bioartificial liver development. The phase I enzymatic activity in hepatic models can be raised exploiting the microfibrillar structure of paper to allow cellular stacking creating hypoxic conditions to induce -like xenobiotic metabolism. Lastly, the paper introduces amalgamation of carbon-based nanomaterials into existing scaffolds in liver tissue engineering.
本文强调了如何使用非传统支架在组织工程构建体中模拟肝脏的重要特征。使用具有类似于天然肝小叶血管排列的脉管系统的脱细胞植物结构或精心设计用于引导细胞排列为肝片或代谢活性的丝混合支架，可以模拟肝小叶组织和代谢分区。在天然纤维结构中，可以增强和维持肝细胞的功能很长一段时间，为生物人工肝的发展铺平道路。通过利用纸张的微纤维结构来允许细胞堆积，创造缺氧条件来诱导类似的异生物质代谢，可以提高肝模型中的一期酶活性。最后，本文介绍了将碳基纳米材料合并到肝组织工程中的现有支架中。
