Chen Jyh-Ping, Lin Tsung-Cheng
Graduate Institute of Biochemical and Biomedical Engineering and Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, ROC.
Biotechnol Prog. 2005 Jan-Feb;21(1):315-9. doi: 10.1021/bp049684v.
The dried fruit from Luffa cylindrica (loofa sponge, LS), which represents a new chitinous source material, was used as a 3-D scaffold for the culture of rat hepatocytes. With the macroporous structure and large pore size (ca. 800 microm) of LS, cell loading to the scaffold should be carried out by dynamic seeding with continuous shaking throughout the seeding period. Hepatocytes attach well to the surface of loofa fibers after seeding and maintain their round shapes. The initial ammonia removal and urea-N synthesis rates of hepatocytes immobilized within LS slightly decreased with increasing cell densities, but their metabolic activities were comparable to or better than those in monolayer culture on tissue culture polystyrene control surfaces. Both urea-N synthesis and albumin secretion rates could be maintained up to 7 days for cells immobilized within LS and spheroid-like cell aggregates could be found after the second day.
来自圆筒丝瓜（丝瓜海绵，LS）的干果，作为一种新的几丁质源材料，被用作大鼠肝细胞培养的三维支架。由于LS具有大孔结构和较大的孔径（约800微米），在整个接种期间应通过持续振荡的动态接种将细胞接种到支架上。接种后肝细胞能很好地附着在丝瓜纤维表面并保持圆形。固定在LS内的肝细胞的初始氨去除率和尿素氮合成率随细胞密度增加略有下降，但其代谢活性与在组织培养聚苯乙烯对照表面上的单层培养相当或更好。固定在LS内的细胞的尿素氮合成率和白蛋白分泌率均可维持7天，且在第二天后可发现类球体样细胞聚集体。
