表达载体pTD1在昆虫无细胞翻译系统中的性能。
Performance of expression vector, pTD1, in insect cell-free translation system.
作者信息
Suzuki Takashi, Ito Masaaki, Ezure Toru, Kobayashi Shinichiro, Shikata Masamitsu, Tanimizu Koji, Nishimura Osamu
机构信息
Life Science Laboratory, Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto 604-8511, Japan.
出版信息
J Biosci Bioeng. 2006 Jul;102(1):69-71. doi: 10.1263/jbb.102.69.
We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.
我们构建了一种用于昆虫无细胞翻译系统的pTD1载体，该载体含有多角体蛋白基因的5'非翻译区（UTR）作为翻译增强子序列。其翻译效率比没有增强子序列的mRNA高约50倍。此外，pTD1载体不仅在昆虫无细胞翻译系统中，而且在小麦胚提取物和兔网织红细胞裂解物系统中都作为一种有效的表达载体发挥作用。
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