Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Biotechnol Adv. 2012 Sep-Oct;30(5):1185-94. doi: 10.1016/j.biotechadv.2011.09.016. Epub 2011 Oct 8.
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
无细胞蛋白质合成已成为一种强大的技术平台，可帮助满足对简单高效蛋白质生产的日益增长的需求。虽然几十年来一直被用作理解转录和翻译的基础研究工具，但最近的进展使得在微尺度到制造规模上合成复杂蛋白质成为可能。蛋白质产率超过每升反应体积产生的克数，批处理反应持续数小时，成本降低了几个数量级，反应规模达到了 100 升的里程碑。这些进展激发了在功能基因组学和结构生物学中合成蛋白质文库、生产个性化药物以及表达病毒样颗粒等方面的新应用。在未来几年，无细胞蛋白质合成有望带来新的工业流程，这些流程对蛋白质生产的时间线要求非常短，同时也为广泛的应用提供了创新方法。
