铜绿假单胞菌PAO1中对三氯乙烯产生正向趋化作用的趋化转导器的鉴定与表征
Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene.
作者信息
Kim Hye-Eun, Shitashiro Maiko, Kuroda Akio, Takiguchi Noboru, Ohtake Hisao, Kato Junichi
机构信息
Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
出版信息
J Bacteriol. 2006 Sep;188(18):6700-2. doi: 10.1128/JB.00584-06.
Abstract
Pseudomonas aeruginosa PAO1 is repelled by trichloroethylene (TCE), and the methyl-accepting chemotaxis proteins PctA, PctB, and PctC serve as the major chemoreceptors for negative chemotaxis to TCE. In this study, we found that the pctABC triple mutant of P. aeruginosa PAO1 was attracted by TCE. Chemotaxis assays of a set of mutants containing deletions in 26 potential mcp genes revealed that mcpA (PA0180) is the chemoreceptor for positive chemotaxis to TCE. McpA also detects tetrachloroethylene and dichloroethylene isomers as attractants.
摘要
铜绿假单胞菌PAO1会被三氯乙烯（TCE）排斥，而甲基接受趋化蛋白PctA、PctB和PctC是对TCE产生负趋化作用的主要化学感受器。在本研究中，我们发现铜绿假单胞菌PAO1的pctABC三突变体被TCE吸引。对一组在26个潜在mcp基因中存在缺失的突变体进行趋化分析表明，mcpA（PA0180）是对TCE产生正趋化作用的化学感受器。McpA还能将四氯乙烯和二氯乙烯异构体检测为引诱剂。
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