Bard H, Prosmanne J
Research Center, Hôpital Sainte-Justine, Montreal, Quebec, Canada.
Pediatrics. 1990 Aug;86(2):193-6.
A study was devised to determine whether levels of fetal hemoglobin (HbF) synthesis are elevated in infants with bronchopulmonary dysplasia (BPD) when compared with the levels of HbF synthesis found in normal control infants. Twelve infants with BPD, whose postconceptional ages ranged from 40 to 62 weeks, were studied. The mean (+/- SD) gestational age and birth weight was 29 +/- 1.9 weeks and 1289 +/- 376 g, respectively. Elevation infants matched for birth weight, gestational age, and postnatal age served as the control subjects. Blood samples were incubated in an amino acid mixture containing [14C]leucine. The adult hemoglobin and HbF were then separated by column chromatography on diethylaminoethyl-Sephadex. The results demonstrated that the mean (+/- SD) level of HbF synthesis in infants with BPD was significantly higher than that in the control infants (42.6 +/- 22.9% vs 18.8 +/- 12.8%; P less than .01). When levels of HbF synthesis in the infants with BPD and the control infants were compared with data previously reported in normal infants, 7 of the 12 infants with BPD, but none of the control infants, were synthesizing amounts of HbF greater than would be expected for their postconceptional age. The results suggest that cardiopulmonary insufficiency could stimulate HbF synthesis during the first year of life as a result of an erythropoietic response to hypoxemia.
设计了一项研究，以确定与正常对照婴儿的胎儿血红蛋白（HbF）合成水平相比，支气管肺发育不良（BPD）婴儿的HbF合成水平是否升高。对12例BPD婴儿进行了研究，其孕龄为40至62周。平均（±标准差）胎龄和出生体重分别为29±1.9周和1289±376克。出生体重、胎龄和出生后年龄匹配的正常婴儿作为对照。将血样在含有[14C]亮氨酸的氨基酸混合物中孵育。然后通过二乙氨基乙基-葡聚糖凝胶柱色谱法分离成人血红蛋白和HbF。结果表明，BPD婴儿的HbF合成平均（±标准差）水平显著高于对照婴儿（42.6±22.9%对18.8±12.8%；P<0.01）。当将BPD婴儿和对照婴儿的HbF合成水平与先前报道的正常婴儿数据进行比较时，12例BPD婴儿中有7例，但对照婴儿中无一例合成的HbF量超过其孕龄预期值。结果表明，心肺功能不全可能由于对低氧血症的红细胞生成反应而在生命的第一年刺激HbF合成。
