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Guide RNAs in kinetoplastid mitochondria have a nonencoded 3' oligo(U) tail involved in recognition of the preedited region.

作者信息

Blum B, Simpson L

机构信息

Department of Biology, University of California, Los Angeles 90024-1606.

出版信息

Cell. 1990 Jul 27;62(2):391-7. doi: 10.1016/0092-8674(90)90375-o.

DOI:10.1016/0092-8674(90)90375-o
PMID:1695552
Abstract

Maxicircle-encoded guide RNAs (gRNAs) for cytochrome b and maxicircle unidentified reading frames 2 and 3 (MURF2 and MURF3) were isolated by hybrid selection and sequenced. All three gRNAs contained nonencoded 3' oligo(U) tails 5-24 nucleotides in length, with a mean length of approximately 15 nucleotides. Secondary structure calculations indicate a functional role of the 3' oligo(U) tail in stabilizing the initial hybrid formed between the gRNA and the preedited mRNA, and allowed the identification of potential mRNA recognition sites for an editing complex. In addition, isolated MURF2 gRNA-II could be 5' capped with [alpha-32P]-GTP and guanylyltransferase, suggesting that at least some gRNAs represent primary transcripts.

摘要

相似文献

1
Guide RNAs in kinetoplastid mitochondria have a nonencoded 3' oligo(U) tail involved in recognition of the preedited region.
Cell. 1990 Jul 27;62(2):391-7. doi: 10.1016/0092-8674(90)90375-o.
2
A model for RNA editing in kinetoplastid mitochondria: "guide" RNA molecules transcribed from maxicircle DNA provide the edited information.动质体线粒体中RNA编辑的一种模型：从大环DNA转录而来的“引导”RNA分子提供编辑信息。
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Kinetoplast DNA minicircles encode guide RNAs for editing of cytochrome oxidase subunit III mRNA.动质体DNA小环编码用于编辑细胞色素氧化酶亚基III mRNA的引导RNA。
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