Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Parasitology. 2021 Sep;148(10):1161-1170. doi: 10.1017/S0031182020002425. Epub 2021 Jan 7.
Complex I (NADH dehydrogenase) is the first enzyme in the respiratory chain. It catalyses the electron transfer from NADH to ubiquinone that is associated with proton pumping out of the matrix. In this study, we characterized NADH dehydrogenase activity in seven monoxenous trypanosomatid species: Blechomonas ayalai, Herpetomonas tarakana, Kentomonas sorsogonicus, Leptomonas seymouri, Novymonas esmeraldas, Sergeia podlipaevi and Wallacemonas raviniae. We also investigated the subunit composition of the complex I in dixenous Phytomonas serpens, in which its presence and activity have been previously documented. In addition to P. serpens, the complex I is functionally active in N. esmeraldas and S. podlipaevi. We also identified 24-32 subunits of the complex I in individual species by using mass spectrometry. Among them, for the first time, we recognized several proteins of the mitochondrial DNA origin.
复合体 I（NADH 脱氢酶）是呼吸链中的第一个酶。它催化从 NADH 到与基质质子泵出相关的泛醌的电子转移。在这项研究中，我们对七种单鞭毛原生动物物种的 NADH 脱氢酶活性进行了表征：Blechomonas ayalai、Herpetomonas tarakana、Kentomonas sorsogonicus、Leptomonas seymouri、Novymonas esmeraldas、Sergeia podlipaevi 和 Wallacemonas raviniae。我们还研究了先前已证明存在和具有活性的二形性植鞭毛虫 Phytomonas serpens 中复合体 I 的亚基组成。除了 P. serpens 之外，复合体 I 在 N. esmeraldas 和 S. podlipaevi 中也具有功能活性。我们还通过使用质谱法在各个物种中鉴定了 24-32 个复合体 I 的亚基。其中，我们首次识别出几种源自线粒体 DNA 的蛋白质。
