Woodworth Bradford A, Lathers Deanne, Neal Jeffery G, Skinner Margaret, Richardson Mary, Young M Rita, Schlosser Rodney J
Department of Otolaryngology-Head and Neck Surgery, Medical University of South, Charleston 29425, USA.
Am J Rhinol. 2006 Jul-Aug;20(4):461-5. doi: 10.2500/ajr.2006.20.2892.
Surfactant-associated proteins (SP) A and D are in the family of collectin proteins that play an integral part in the innate defense system. SP-A and SP-D expression and function are altered in a variety of inflammatory and infectious diseases of the lungs, such as asthma, allergies, and cystic fibrosis. Our prior studies are the first to identify the presence of these proteins in the human sinonasal cavity. The objective of this study was to immunolocalize SP-A and SP-D in human sinonasal tissue.
Sinonasal mucosal biopsies were performed in patients with various forms of chronic hyperplastic rhinosinusitis with nasal polyposis and nondiseased mucosa from patients undergoing transsphenoidal hypophysectomy. (n = 10) Immunolocalization of surfactant proteins was performed with antibodies to SP-A and SP-D using immunoperoxidase staining technique. Isotype-negative controls were performed on all specimens.
Analyses of mucosal biopsy specimens from human sinonasal tissue reveals staining within respiratory and intermediate (metaplastic)-type surface epithelium. In addition, staining was intense in the submucosal ductal epithelium of the seromucinous glands. These properties appear to be consistent regardless of disease state and location within the sinuses.
This is the first study to immunolocalize SP-A and SP-D in sinonasal human mucosa. These are secreted proteins that are intricately involved in innate immunity in the lungs. Their secretion in the upper airway indicates that future studies may allow manipulation of these proteins and development of novel treatments for sinonasal pathology.
表面活性物质相关蛋白（SP）A和D属于凝集素蛋白家族，在先天防御系统中发挥着不可或缺的作用。在多种肺部炎症和感染性疾病，如哮喘、过敏和囊性纤维化中，SP - A和SP - D的表达及功能会发生改变。我们之前的研究首次在人类鼻窦腔中发现了这些蛋白的存在。本研究的目的是在人类鼻窦组织中对SP - A和SP - D进行免疫定位。
对患有各种形式的慢性增生性鼻窦炎伴鼻息肉的患者以及接受经蝶垂体切除术患者的正常鼻窦黏膜进行活检。（n = 10）使用免疫过氧化物酶染色技术，用抗SP - A和SP - D的抗体对表面活性物质蛋白进行免疫定位。对所有标本进行同型阴性对照。
对人类鼻窦组织黏膜活检标本的分析显示，在呼吸型和中间型（化生型）表面上皮中有染色。此外，在浆液黏液性腺的黏膜下导管上皮中染色强烈。无论疾病状态和鼻窦内的位置如何，这些特性似乎都是一致的。
这是第一项在人类鼻窦黏膜中对SP - A和SP - D进行免疫定位的研究。这些是在肺部先天免疫中复杂参与的分泌蛋白。它们在上呼吸道中的分泌表明，未来的研究可能会实现对这些蛋白的调控，并开发出针对鼻窦疾病的新疗法。
