白细胞介素4诱导人B细胞转换为产生IgE时种系ε转录本的结构与表达
Structure and expression of germline epsilon transcripts in human B cells induced by interleukin 4 to switch to IgE production.
作者信息
Gauchat J F, Lebman D A, Coffman R L, Gascan H, de Vries J E
机构信息
DNAX Research Institute, Palo Alto, California 94304.
出版信息
J Exp Med. 1990 Aug 1;172(2):463-73. doi: 10.1084/jem.172.2.463.
Abstract
Interleukin 4 (IL-4)-induced IgE production coincides with the appearance of the 2.2-kb productive epsilon-mRNA, but is preceded by synthesis of a 1.7-kb epsilon-RNA. Analysis of cDNA copies of the 5' end of this RNA indicated that the 1.7-kb epsilon-RNA is a germline epsilon immunoglobulin heavy chain transcript with an exon mapping 5' to the switch region. Transcription through switch regions has been implicated in the control of class switching. However, IL-4 or cloned CD4+ T cells were able to induce germline epsilon transcripts without inducing IgE synthesis, for which both signals were required. These results indicate that induction of human germline epsilon-RNA does not necessarily result in IgE synthesis, and that additional regulatory mechanisms are involved in class switching.
摘要
白细胞介素4（IL-4）诱导的IgE产生与2.2 kb有功能的ε-信使核糖核酸（mRNA）的出现同时发生，但在此之前会先合成1.7 kb的ε-核糖核酸（RNA）。对该RNA 5'端的互补脱氧核糖核酸（cDNA）拷贝进行分析表明，1.7 kb的ε-RNA是一种种系ε免疫球蛋白重链转录本，其一个外显子位于转换区的5'端。通过转换区的转录与类别转换的控制有关。然而，IL-4或克隆的CD4 + T细胞能够诱导种系ε转录本，但不诱导IgE合成，而这两个信号都是必需的。这些结果表明，人源种系ε-RNA的诱导不一定会导致IgE合成，并且在类别转换中还涉及其他调节机制。
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