Li Yun, Deng Xi-Ping, Kwak Sang-Soo, Tanaka Kiyoshi
State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Research Center of Water and Soil Conservation and Ecological Environment, Chinese Academy of Sciences, Yangling 712100, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Aug;32(4):451-7.
Two strains, Cu/Zn SOD and APX gene transferred sweet potato (TS) and non-transgenic sweet potato (Ipomoea batatas L.) (NT), were used as experimental materials to study the drought tolerance under three different degrees of water stress: 0, -0.44 MPa, -0.78 MPa. The results showed that activities of Cu/Zn SOD and APX increased under -0.44 MPa and decreased under -0.78 MPa (Fig. 2), P(n), G(s) and leaf water content decreased, C(i) increased, then decreased under water stress (Fig. 6), but under the same PEG concentration all these indexes in the TS were higher than those in NT. The accumulation of H(2)O(2) and O(-*)(2) (Fig. 1) increased the degree of lipid peroxidation of the plasma membrane (Fig. 4), prompted the accumulation of MDA (Fig. 3), and the accumulation of TS always lower than the NT at the same PEG concentration. All the results showed that the transgenic sweet potato has a stronger ability to clean up active oxygen than the non-transgenic one, and it can keep a higher leaf water content and P(n) under water stress, so it has a stronger tolerance to drought.
以转铜/锌超氧化物歧化酶(Cu/Zn SOD)和抗坏血酸过氧化物酶(APX)基因甘薯(TS)及非转基因甘薯(Ipomoea batatas L.)(NT)两个品系为试验材料,研究其在0、-0.44 MPa、-0.78 MPa三种不同程度水分胁迫下的耐旱性。结果表明,在-0.44 MPa水分胁迫下,Cu/Zn SOD和APX活性升高,而在-0.78 MPa水分胁迫下则降低(图2);水分胁迫下,净光合速率(P(n))、气孔导度(G(s))和叶片含水量降低,胞间二氧化碳浓度(C(i))先升高后降低(图6),但在相同聚乙二醇(PEG)浓度下,TS的各项指标均高于NT。过氧化氢(H(2)O(2))和超氧阴离子(O(-*)(2))的积累(图1)增加了质膜脂质过氧化程度(图4),促使丙二醛(MDA)积累(图3),且在相同PEG浓度下,TS的积累量始终低于NT。所有结果表明,转基因甘薯清除活性氧的能力强于非转基因甘薯,且在水分胁迫下能保持较高的叶片含水量和P(n),因此具有较强的耐旱性。