Al-Nazhan Saad, Al-Nasser Afaf
Department of Restorative Dental Science, Division of Endodontics, College of Dentistry at King Saud University, Riyadh, Saudi Arabia.
J Contemp Dent Pract. 2006 Sep 1;7(4):37-44.
The viability of the periodontal ligament (PDL) cells is critical for successful healing of replanted avulsed teeth. Viability is primarily dependent on the duration of the extra-alveolar time and storage medium used to preserve teeth. Several storage media have been suggested but milk ranks highest. It would be desirable to evaluate other media as a suitable alternative for milk. The purpose of this study was to determine the viability of human PDL fibroblasts and their morphology after storage in different types of contact lens solutions.
PDL fibroblasts were cultured from a healthy extracted impacted human tooth and exposed to Bausch and Lomb (Renu), Ciba Vision (Titmus), and Alcon (Opti-free) contact lens solutions. Eagle's minimal essential medium served as control. The experiment was performed in plastic tissue culture clusters containing 24 wells. The PDL fibroblasts were grown in each well for three days. On the day of the experiment the culture medium was decanted, the cells were washed with phosphate buffered saline solution (PBS), and 1 ml of the tested solution was placed in each culture well. All tissue culture clusters were incubated at 37 degrees C in 5% CO2 and 95% air for one, four, and 24 hrs. At the end of the incubation period, the cells were fixed and prepared for scanning electron microscope (SEM) examination.
The results indicated Renu and Opti-free solutions were superior to Titmus solution in terms of their capacity to maintain the viability and normal morphology of PDL fibroblasts.
Contact lens solution is a good storage medium to maintain the viability of PDL fibroblasts for a short-term period.
牙周膜（PDL）细胞的活力对于再植脱位牙的成功愈合至关重要。活力主要取决于牙槽外时间的长短以及用于保存牙齿的储存介质。已经提出了几种储存介质，但牛奶的效果最佳。评估其他介质作为牛奶的合适替代品是很有必要的。本研究的目的是确定人PDL成纤维细胞在不同类型隐形眼镜护理液中储存后的活力及其形态。
从一颗健康拔除的阻生人类牙齿中培养PDL成纤维细胞，并将其暴露于博士伦（润明）、视康（缇摩斯）和爱尔康（傲滴）隐形眼镜护理液中。伊格尔最低限度基本培养基用作对照。实验在含有24个孔的塑料组织培养板中进行。PDL成纤维细胞在每个孔中培养三天。在实验当天，倒出培养基，用磷酸盐缓冲盐水溶液（PBS）洗涤细胞，并在每个培养孔中加入1毫升测试溶液。所有组织培养板在37℃、5%二氧化碳和95%空气的环境中孵育1小时、4小时和24小时。孵育期结束时，固定细胞并准备进行扫描电子显微镜（SEM）检查。
结果表明，就维持PDL成纤维细胞活力和正常形态的能力而言，润明护理液和傲滴护理液优于缇摩斯护理液。
隐形眼镜护理液是一种在短期内维持PDL成纤维细胞活力的良好储存介质。
