Huang S C, Remeikis N A, Daniel J C
Department of Endodontics, University of Illinois at Chicago, 60612-7212, USA.
J Endod. 1996 Jan;22(1):30-3. doi: 10.1016/S0099-2399(96)80233-0.
Periodontal ligament (PDL) cells cultured from healthy extracted human teeth were exposed to milk, Alcon Opti-Free contact lens solution, K-Mart contact lens solution, saline, and Hank's balanced salt solution. The appearance and rate of loss of the cells from the culture dishes were recorded over time at both room temperature (20 degrees C) and 4 degrees C. The results indicated that saline was superior to either of the contact lens solutions in its ability to maintain the vitality of the PDL cells. Milk at 4 degrees DC provided good short-term viability , but cells did not remain attached after 48 h. At 20 degrees C, however, milk resulted in a 24.4% retention of cells after 72 h. Hank's balanced salt solution was the best storage media, with 46.8% of sells remaining attached after 72 h of exposure. This study supports milk as a good short-term storage medium for maintaining the vitality of PDL cells in vitro.
从健康拔除的人牙中培养的牙周膜（PDL）细胞被暴露于牛奶、爱尔康Opti-Free隐形眼镜护理液、凯马特隐形眼镜护理液、生理盐水和汉克平衡盐溶液中。在室温（20摄氏度）和4摄氏度下，随着时间的推移记录培养皿中细胞的外观和损失率。结果表明，生理盐水在维持PDL细胞活力方面优于任何一种隐形眼镜护理液。4摄氏度的牛奶能提供良好的短期活力，但48小时后细胞不再附着。然而，在20摄氏度时，72小时后牛奶使24.4%的细胞得以保留。汉克平衡盐溶液是最佳的储存培养基，暴露72小时后有46.8%的细胞仍附着。本研究支持牛奶作为一种在体外维持PDL细胞活力的良好短期储存培养基。
