Ozan Fatih, Polat Zübeyde Akin, Er Kürsat, Ozan Ulkü, Değer Orhan
Department of Oral and Maxillofacial Surgery, School of Dentistry, Cumhuriyet University, Sivas, Turkey.
J Endod. 2007 May;33(5):570-3. doi: 10.1016/j.joen.2006.12.021. Epub 2007 Feb 23.
Propolis is a multifunctional material used by bees in the construction and maintenance of their hives. Propolis possesses several biologic activities such as anti-inflammatory, antibacterial, antioxidant, antifungal, antiviral, and tissue regenerative, among others. The purpose of this study was to determine the ability of propolis to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagles Medium (DMEM). Cultures were subjected to 10% propolis solution, 20% propolis solution, long-shelf life light milk with lower fat content (milk), Hank's Balanced Salt Solution, tap water as the negative control, and DMEM as the positive control. Tissue culture plates were incubated with experimental media at 37 degrees C for 1, 3, 6, 12, or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was accomplished by using one-way analysis of variance complemented by the Tukey test. The level of significance was 5% (p<0.05). The results showed that 10% propolis was a more effective storage medium than other groups. In conclusion, propolis can be recommended as a suitable transport medium for avulsed teeth.
蜂胶是蜜蜂用于建造和维护蜂巢的多功能材料。蜂胶具有多种生物活性，如抗炎、抗菌、抗氧化、抗真菌、抗病毒和组织再生等。本研究的目的是确定蜂胶作为临时储存介质维持离体牙牙周膜（PDL）细胞活力的能力。从健康的第三磨牙获取PDL细胞，并在杜氏改良伊格尔培养基（DMEM）中培养。培养物分别用10%蜂胶溶液、20%蜂胶溶液、低脂肪含量的长期保存轻质牛奶（牛奶）、汉克平衡盐溶液、自来水作为阴性对照，以及DMEM作为阳性对照处理。将组织培养板与实验培养基在37℃孵育1、3、6、12或24小时。通过台盼蓝排斥法评估PDL细胞活力。数据的统计分析采用单因素方差分析并辅以Tukey检验完成。显著性水平为5%（p<0.05）。结果表明，10%蜂胶是比其他组更有效的储存介质。总之，蜂胶可被推荐为离体牙合适的运输介质。
