Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 150 Sungan-ro, Kangdong-gu, Seoul, 05355, South Korea.
Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, South Korea.
Eur J Clin Microbiol Infect Dis. 2019 Dec;38(12):2229-2233. doi: 10.1007/s10096-019-03663-3. Epub 2019 Aug 2.
The performance of BACT/ALERT FA and FN PLUS (FA PLUS and FN PLUS) blood culture bottles with the BACT/ALERT VIRTUO (bioMérieux, Inc., Durham, NC) and BD BACTEC Plus Aerobic and Anaerobic (BD Aerobic and BD Anaerobic) blood culture bottles with the BD BACTEC FX (BD Diagnostics, Sparks, MD) for antimicrobial neutralization at peak serum concentration was evaluated. The following antibiotic agents and microbial strains were used: ampicillin, cefepime, cefotaxime, gentamicin, levofloxacin, meropenem, piperacillin-tazobactam, and vancomycin; methicillin-sensitive Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Bacteroides fragilis. The detection rate of FA PLUS bottles was 69.1% (259/375) and that of BD Aerobic bottles was 75.5% (283/375) (p = 0.050). In the case of anaerobic culture, the overall detection rate of FN PLUS bottles was 77.0% (231/300) and that of BD Anaerobic bottles was 71.3% (214/300) (p = 0.113). The time to detection (TTD) from aerobic culture was 2.8 h shorter in FA PLUS bottles (12.4 h) compared to BD Aerobic bottles (15.2 h) (p < 0.001). And the TTD from anaerobic culture was 1.6 h shorter in FN PLUS bottles (18.1 h) compared to BD Anaerobic bottles (19.7 h) (p = 0.061). The FA PLUS bottles exhibited a lower detection rate compared to BD Aerobic bottles, while FN PLUS bottles showed a higher detection rate compared to BD Anaerobic bottles. The BACT/ALERT VIRTUO system exhibited shorter TTD compared to the BD BACTEC FX system for both aerobic and anaerobic cultures.
评估了 BACT/ALERT FA 和 FN PLUS（FA PLUS 和 FN PLUS）血培养瓶与 BACT/ALERT VIRTUO（bioMérieux，Inc.，Durham，NC）和 BD BACTEC Plus 需氧和厌氧（BD Aerobic 和 BD Anaerobic）血培养瓶与 BD BACTEC FX（BD Diagnostics，Sparks，MD）在峰值血清浓度下对抗生素中和的性能。使用了以下抗生素药物和微生物菌株：氨苄西林、头孢吡肟、头孢噻肟、庆大霉素、左氧氟沙星、美罗培南、哌拉西林他唑巴坦和万古霉素；甲氧西林敏感金黄色葡萄球菌、鲍曼不动杆菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和脆弱拟杆菌。FA PLUS 瓶的检测率为 69.1%（259/375），BD Aerobic 瓶的检测率为 75.5%（283/375）（p=0.050）。在厌氧培养的情况下，FN PLUS 瓶的总体检测率为 77.0%（231/300），BD Anaerobic 瓶的检测率为 71.3%（214/300）（p=0.113）。FA PLUS 瓶的需氧培养 TTD 比 BD Aerobic 瓶短 2.8 小时（12.4 小时）（p<0.001）。FN PLUS 瓶的厌氧培养 TTD 比 BD Anaerobic 瓶短 1.6 小时（18.1 小时）（p=0.061）。FA PLUS 瓶的检测率低于 BD Aerobic 瓶，而 FN PLUS 瓶的检测率高于 BD Anaerobic 瓶。与 BD BACTEC FX 系统相比，BACT/ALERT VIRTUO 系统在需氧和厌氧培养时的 TTD 都更短。
