Charrier Anne, Candoni Nadine, Liachenko Natalia, Thibaudau Franck
CRMCN, CNRS, Université de la Méditerranée, Marseille, France.
Biosens Bioelectron. 2007 Apr 15;22(9-10):1881-6. doi: 10.1016/j.bios.2006.07.024. Epub 2006 Sep 7.
A 2D colorimetric DNA sensor is reported based on the 2D aggregation of oligonucleotide-modified gold nanoparticle probes resulting from the molecular hybridization between these latest and their complementary single stranded DNA targets. To increase their mobility the nanoparticles are adsorbed on a fluid lipid bilayer, itself supported on a substrate. The hybridization between the target and the mobile nanoparticle probes creates links between the nanoparticles resulting in the formation of nanoparticle aggregates in the plane of the substrate. This aggregation is detected using a new method based on the selective desorption of non-aggregated nanoparticles. The addition of dextran sulfate induces the substitution of non-aggregated gold nanoparticles while aggregated ones are stable on the substrate. We show that this detection method is highly specific and allows the detection of DNA mismatches and damages.
报道了一种基于寡核苷酸修饰的金纳米颗粒探针二维聚集的二维比色DNA传感器，这种聚集是由这些探针与它们的互补单链DNA靶标之间的分子杂交产生的。为了增加其流动性，纳米颗粒被吸附在流体脂质双层上，而脂质双层本身又支撑在基底上。靶标与可移动的纳米颗粒探针之间的杂交在纳米颗粒之间形成连接，导致在基底平面上形成纳米颗粒聚集体。使用一种基于非聚集纳米颗粒选择性解吸的新方法检测这种聚集。添加硫酸葡聚糖会导致非聚集金纳米颗粒被取代，而聚集的纳米颗粒在基底上保持稳定。我们表明，这种检测方法具有高度特异性，能够检测DNA错配和损伤。
